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What kind of sample cleanup does solid-phase extraction accomplish in the analysis of caffeine and theobromine in chocolate?
Introduction:
Extraction is the process of separation of the molecules. There are two types of extraction procedures like solid phase extraction and liquid phase extraction. The distribution of solute depends upon the partition theory.
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- Describe chromatography analysis, the two essential phases used in paper chromatography and provide an example.Column chromatography is also very useful for separating mixtures of organic compounds that are not coloured. Briefly explain how you could use column chromatography for the separation of a colourless mixture, and how you could identify your separated compounds.Differentiate normal phase chromatography from reverse phase chromatography. From which of the two governs the video demonstration in TLC and paper chromatography? How do you say so?
- Amino acids and fatty acids do not readily elute from gas chromatography columns even at temperatures above 200 degrees Celsius. What can be done to these biomolecules to allow gas chromatographic analysis?What further testing should be done to extract and compare the composition of food dyes used In two types of candies.(except in paper chromatography)Explain the following Chromatography (for drug analysis) Thin-layer chromatography (TLC) Gas chromatography (GC) Liquid chromatography (LC) (i.e, high performance liquid chromatography or HPLC)