What will be happen if you mix; 1ml of 5% solution of albumin and 5 mL of 95% ethanol 1ml of 5% solution of gelatin and 5 mL of 95% ethanol 1ml of 5% solution of peptone and 5 mL of 95% ethanol Mix and observe the formation of precipitate.
Q: Why is the order of separation based on size opposite for gel filtration and gel electrophoresis,…
A: Gel filtration and gel electrophoresis are biochemical techniques used for the separation of…
Q: Asnwer the given question a and b, DO NOT JUST COPY PASTE IMAGES FROM GOOGLE
A: FRAP is the Fluorescence Recovery After Photobleaching. It is the technique that used the…
Q: If you run the Lugol's test on an unknown solution and you get a black color at the end, what does…
A: The use of Lugol's iodine reagent is used to differentiate starch and glycogen from other…
Q: During two-dimensional gel electrophoresis, proteins are separatedbased ona. their net charge at a…
A: Gel electrophoresis is a separation technique used in biotechnology and other research domains to…
Q: Gel used in electrophoresis is/are: a. Agarose gel b. Polyacrylamide plain gel c. Polyacrylamide SDS…
A: Electrophoresis is a method used to separate mixtures of RNA , DNA or proteins according to their…
Q: how many ml of sterile water injection must be mixed with two 4 ml vials of sterile hypertonic NaCl…
A: Tonicity refers to the ability of a solvent to cause cells to shrink or swell. A semipermeable…
Q: Four proteins Cytochrome C (pI=10.2) Myoglobin (pI=7.2), Hemoglobin (pI = 6.8) and Serum Albumin…
A: Introduction: Proteins are important macromolecule that helps in the structure and function of the…
Q: Define about sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)? Explain…
A: The process aims at the separation along with the analysis of the macromolecules that include…
Q: A plant isolate was subjected to qualitative tests to determine the presence of macromolecules.…
A: Biological macromolecules are constituted of proteins , nucleic acids (DNA or RNA), carbohydrates…
Q: In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?
A: Gel-filtration chromatography is a type of partition chromatography that is used to separate…
Q: Predict the results you would get if your Cytosine DNA sample was inadvertently contaminated with…
A: DNA is a nucleic acids and polymer of nucleotides linked by phosphodiester bonds. It is composed of…
Q: Why does water separate out from a gel after freezing?
A: On freezing of gel some damage occur to gel. damage occur to polymer and colloids that binds the gel…
Q: In size exclusion chromatography, which component will elute last in the set up? Group of answer…
A: Size exclusion chromatography is also known as gel filtration chromatography (GFC) or gel permeation…
Q: Why did the gelatin tubes have to be moved to 4C before analysis? a. If gelatinase was produced the…
A: Gelatin is a protein derivative obtained from animal collagen. A gelatin test is performed in…
Q: How is a DNA viewed on an agarose gel?
A: DNA stands for deoxyribonucleic acid. On an agarose gel, DNA is stained with the ethidium bromide.…
Q: In SDS page, the ________ proteins will move through the gel fastest and first, and in size…
A: SDS-PAGE is biochemistry technique used to separate proteins based upon the relative mobility of…
Q: Identify the approximate amount of the visible band II. Describe the trouble shooting problem from…
A: This image is showing gel electrophoresis and the separation of DNA fragments. Gel electrophoresis…
Q: What characteristic of agarose makes it useful for electrophoresis? It is more dense than water…
A: Introduction : Agarose gel electrophoresis is the most useful and common technique that is used to…
Q: A plant isolate was subjected to qualitative tests to determine the presence of macromolecules.…
A: Qualitative tests detect the presence of specific molecules in a sample solution. There are…
Q: When preparing cells for karyotyping, in which of the following solutions should the cells be…
A: Karyotyping In this technique, the chromosomes are arrested in the metaphase stage of cell division…
Q: To release DNA from cells, detergent is used to disrupt the membranes of the cell and nucleus. What…
A: Membranes are primarily composed of a bilayer of lipids(fats). In addition to lipids, there are…
Q: In a lab experiment, there was yeast added to four different test tubes. One tube has water, one…
A: Answer Yeast belongs to fungus group The four different test tubes will show different results…
Q: What is the use of color reaction tests for proteins in diagnosing diseases? Give an example too!
A: Diagnostics, which can diagnose illness, disorders, or infections, is quickly becoming a critical…
Q: If you have an absorbance of 2.0 mL of stock dye, what kind of a dilution is needed to get it to…
A: Biochemistry is the science of study of the internal biochemical processes of organisms. The…
Q: Which among the following demonstrates the difference between Native Polyacrylamide Gel…
A: PAGE or polyacrylamide gel electrophoresis is a separation technique under the influence of…
Q: Three cell membrane samples: M, C, and S were fluorescently labeled and were subsequently…
A: Introduction: The cell membrane, also known as the plasma membrane, is a semi-permeable membrane…
Q: In extraction of albumin in egg white using 100-ml water, what does albumin look like? Also, what do…
A: In extraction of albumin in egg white and placed in water some reactions are occur..
Q: Distinguish between each of the following pairs of substances by giving both the test and the…
A: Amino acids are molecules made up of a carboxyl group, an amino group, a variable R group, and a…
Q: What are the characteristics of an acidic dye? Of a basic dye? Indicate whether methylene blue is an…
A: Dyes are colored compounds that are used as coloring agents in laboratories, textile industry, etc.…
Q: zation occurs predominantly at the minus (-) ends of microtubules. Polymerization is favored…
A: Cell biology is study of the cell and how they grow and how they communicate with each other. Cell…
Q: What will happen to the gelatin if you put cooked pineapple and fresh pineapple in a Bromelain test?
A: Gelatin is made from disintegrating animal hides, boiled pulverised bones, and cattle and pig…
Q: Would a red blood cell placed in a 200mM sucrose solution lyse?
A: Osmosis is a process in which water molecules moves from the low concentration of solute to the…
Q: Which of the following DNA sequences will travel the shortest amount down a gel? A ATTGCAGGCCT B…
A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…
Q: How many cells would you be plating if you pipetted 200uL into your dish from a 125 x 104 cells/mL…
A: The question is of calculation with keeping the units in our mind .
Q: Based on the results of the qualitative tests, what amino acids are confirmed to be present in…
A: The amino acid detected in sakaguchi test is arginine. Arginine has a guanidine group in its side…
Q: Identify the approximate amount of the visible band II. Describe the trouble shooting problem from…
A: Approximate amount of the visible band is between 50 to 75.
Q: Which of the following statements is TRUE about the band of molecules labeled X ( close to where it…
A: Agarose gel electrophoresis is carried out to separate DNA fragments under the influence of an…
Q: in paper chromatography what is the basis of pigments separate?
A: Paper chromatography is a technique in which different types of pigments can be separated from each…
Q: Hi! can you show the solution through a punnet square?
A: Introduction Blood groups are determined by the presence or absence of antibodies and inherited…
Q: A 25.0 mL aliquot was taken from a 500.0 mL sample of tonic water containing an unknown amount of…
A: Quinine is generally helped to treat malaria that leads by Plasmodium falciparum. This parasite that…
Q: n prophase genetic material condenses and coils into what form of genetic material?
A: Prophase is the stage of mitosis where the duplicated genetic material carried in the nucleus of a…
Q: Draw a hydropathy plot and a 2D protein cartoon for a protein that passes through the lipid bilayer…
A: Based on the polarities of the side chains of amino acids they can be arranged in a hydrophobicity…
Q: If the potato cells were hypotonic to the surrounding solution, the surrounding solution itself…
A: * Hypotonic solution means the solution will have lower concentration of solutes than water. *…
Q: Three cell membrane samples: M, C, and S were fluorescently labeled and were subsequently…
A: Fluorescent Recovery After Photobleaching (FRAP) is a technique used to test the mobility…
Q: What is the purpose of each of the following ingredients when present in an homogenization buffer…
A: Homogenization Homogenization is the process of reducing size of the bigger particles and also…
Q: When the potato cube is surrounded by a hypotonic solution what change in mass occurs in the potato…
A: Tonicity is the phenomenon that takes into consideration the solute concentration. Osmoregulation…
Q: Why Are Cells Small
A: According to bartleby guidelines when multiple questions are posted we are allowed to answer the…
What will be happen if you mix;
1ml of 5% solution of albumin and 5 mL of 95% ethanol
1ml of 5% solution of gelatin and 5 mL of 95% ethanol
1ml of 5% solution of peptone and 5 mL of 95% ethanol
Mix and observe the formation of precipitate.
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- The following gel was obtained using the Sanger method. What is the DNA sequence and what does the arrow represent?In an experiment to investigate membrane integrity, beet root sections were subjected to different chemicals. Membrane damage was estimated by the color intensity of leaked pigment. Five replicates of each treatment were prepared. Complete the following table with the absorbance values. Calculate the mean (average) and the standard Table 1: Absorbance readings of pigment leaked from damaged cells treated with two different chemicals. Replicate # Absorbance at 460 nm Chemical 2 Chemical 6 1 0.357 0.392 2 0.576 0.395 3 0.334 0.385 4 0.197 0.399 5 0.334 0.403 Average SD Calculate the Coefficient of Variance (see the appendix) for the readings of each chemical: Evaluate the precision of each and state which one is more precise:Based on the results of the qualitative tests, what amino acids are confirmed to be present in gelatin, casein, and albumin respectively? refer to the attached image
- For a TLC experiment, what is the purpose of the pentane extraction of spinach juice? Why wouldn’t we want to spot an aqueous solution onto the silica gel?Explain how to prepare 2 ml of a solution with a concentration of 1 μg/5ml from a stock solution of a DNA sample with a concentration of 0.1 mg/ml.Predict the results you would get if your Cytosine DNA sample was inadvertently contaminated with the Thymine DNA sample both with Potassium permanganate. Which colour would be shown? How could you experimentally confirm your prediction?
- A piece of DNA is cut into four fragments as shown below. A solution containing the four fragments is placed in a single well at the top of an agarose gel. Using the information given below, draw (below the well) how you think the fragments will be aligned on the gell following electrophoresis. Label each fragment with its corresponding letter. Each band on the gel will be the same width, equal to the width of the well at the top of the gell. These should be in one lane. Do smaller or larger (pb) fragments migrate the furthest? Why?What is acetic orcein and what structure does it stain? Orcein contains different phenazones, which, at acid pH binds with negatively charged (phosphate) groups of DNA and possibly also interacts hydrophobically with chromatin Orcein contains different phenazones, which, at basic pH binds with negatively charged (phosphate) groups of protein and possibly also interacts hydrophobically with chromatin Orcein contains different phenazones, which, at acid pH binds with negatively charged (phosphate) groups of DNA and possibly also interacts hydrophilically with chromatin Orcein contains different phenazones, which, at acid pH with negatively charged (phosphate) groups of protein and possibly also interacts hydrophobically with chromatinWhy is the order of separation based on size opposite for gel filtration and gel electrophoresis, even though they often use the same compound to form the matrix?
- When run under nondenaturing conditions (without SDS or β-mercaptoethanol), nativepolyacrylamide gel electrophoresis (PAGE) allows proteins to maintain biological activity, so the GFP band should fluoresce when exposed to UV light. How do the staining patterns of gels run in this way differ from the staining patterns of gels run using SDS-PAGE?For various species or portions of organisms, different stains have different affinities. Explain this assertion using the example of hematoxylin and eosin staining.Hand draw the expected gel result from the graphical data of three extracted RNA samples (in Figure 1a, 1b, & 1c) in Nanodrop spectrophotometer. The 3 graphical data should be in 1 agarose gel Provide complete gel label descriptions (ladder, lanes, etc.).