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If you run the Lugol's test on an unknown solution and you get a black color at the end, what does this result tell you?
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- If you have an absorbance of 2.0 mL of stock dye, what kind of a dilution is needed to get it to 1.0? How do you go about this dilution and how do you calculate?in paper chromatography what is the basis of pigments separate?The following strip of chromatography paper has been placed in a polar solvent Which pigments the most polar?
- Using my chart can you help me with the following question. Based on your result, what color do you expect Benedict’s solution to turn in the presence of a simple sugar? Explain what information from your results suggests that Benedict’s solution will turn that particular color when testing a simple sugar?What is the driving force behind paper chromatography?In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?
- Why is it important that the agarose is exposed to ethidium bromide, which is either added to the gel itself or mixed to the staining solution? Explain the principle behind its use.In the assay of citric acid, what do you think about the nature of solution sample ? a) Before titration and b) After titration.In gel electrophoresis and column chromatography, why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?