What would the gc chromatogram look like when 6.65 g NaBr, 3.78 g NaCl, 7.5 mL of DI water and 5 mL of 1‐butanol are added together then 6 mL of concentrated sulfuric acid added.
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What would the gc chromatogram look like when 6.65 g NaBr, 3.78 g NaCl, 7.5 mL of DI water and 5 mL of 1‐butanol are added together then 6 mL of concentrated sulfuric acid added.
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- In a chromatographic analysis, it was found out that distance travelled by the alcohol solvent is 4 cm away from the finish line. The distance travelled from the starting line to finish line is 40 cm. The first amino acid, AA1 travelled 6 cm behind the distance travelled by the alcohol. The second amino acid, AA2 travelled 12 cm short from the solvent front while the third amino acid, AA3 travelled 8 cm ahead of AA2. Calculate: Rf of AA1 ____ Rf of AA2____ Rf of AA3____ (Rf= Retention factor)Upon screening with TLC (made of silica gel) chromatography using hexane/chloroform (3:1) as eluting solution, 3 spots were detected corresponding to a (A) polyphenol, a (B) tannin and an (C) alkaloid. Write the letters of your answers on the spots in the chromatogram shown at the right.Upon screening with TLC (made of silica gel) chromatography using hexane/chloroform (3:1) as eluting solution, 3 spots were detected corresponding to a (A) polyphenol, a (B) tannin and an (C) alkaloid. Write the letters of the on the spots in the chromatogram shown at the right.
- 1. How does chromatography separate the components of leave samples? 2. Can you use chromatography to purify a mixture? why? 3. What is Rf value and its significance in chromatography?How does the chromatogram change when methanol:water (90:10) is used as the mobile phase compared to a 100% methanol run?4) If the classification of the chromatography run is a reverse-phase chromatography, discuss the relative polarities of caffeine, aspartame and benzoate.
- A mixture of seven amino acids (glycine, glutamate, leucine, lysine, alanine, isoleucine, and aspartate) is separated by chromatography. Explain why only six spots show up when the chromatographic plate is coated with ninhydrin and heated.According to the label, the commercial glass cleaner Windex contains 8 ingredients; however, upon GC analysis only 7 compounds could be identified. Sodium dodecylbenzene sulfonate could not be located in the chromatogram. Why might this be the case? A) The sodium dodecylbenzene sulfonate evaporated before analysis could take place B) The sodium dodecylbenzene sulfonate has a very high boiling point C) The sodium dodecylbenzene sulfonate elutes at the same exact time as another component of the mixture Answers A & B Answers A & C Answers B & C Answers A, B, & CWhich of the following statement is false? a. Component with the strong attraction to the support move more slowly than component with weak attraction. b. Chromatography technique used to separate only colored compounds. c. On TLC, single spot does not mean or guarantee a single substance (compound). d. Iodine vapor and UV- lamp are methods used for visualization uncolored compounds.
- A mixture contains the following compounds: octane, octanoic acid, 1-chlorooctane and octanal. Arrange the following compounds in INCREASING Rf if the mixture underwent paper chromatography with the same solvent system used in the experimentThe following questions regard the chromatograms shown below: Which column gives a greater selectivity factor for compound X and compound Y? Explain your answer.Separation of Amino Acids by Thin Layer Chromatography Lab Questions. Thank you for your help on these questions!