When gel electrophoresis is undertaken, one well always contains DNA-marker molecules. The purpose of these markers is to Question 30 options: help visualize the DNA indicate when the DC current is turned on serve as a guide to the length of the fragments in the other wells indicate when the DNA has completely crossed the gel serve as a channel for ethidium bromide

When gel electrophoresis is undertaken, one well always contains DNA-marker molecules. The purpose of these markers is to Question 30 options: help visualize the DNA indicate when the DC current is turned on serve as a guide to the length of the fragments in the other wells indicate when the DNA has completely crossed the gel serve as a channel for ethidium bromide

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter10: Biotechnology

Section: Chapter Questions

Problem 2RQ: n gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules...

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n gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules _______. a. are from different organisms b. have different lengths c. have different nucleotide compositions d. have different genes
Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.
Which of the following best describes the process of DNA seqencing. a. DNA is seperated on a gel and the different bands are labled with flouroscent nucleotides and scanned with a laser. b. A laser is used to flurorescently label the nucleotides present with in the DNA , the DNA is run on a gel and then the DNA is droken into fragments c. Nucleotides are scanned with a laser and incrprorated into the DNA that has been seperated on a gel and then DNA is amplified with PCR. d. fragments of DNA are produced in a reaction that lables them with any of four different fluroscent dyes and the fragmented then are run on a gel and scanned with laser e. DNA is broken down into its constituents nucleotides and the nucleotides are then run on a gel and purified with a laser
This piece of DNA is cut by EcoRI, the resulting fragments are separated by gel electrophoresis, and the gel is stained with ethidium bromide. Draw a picture of the bands that will appear on the gel.
Which of the following is TRUE with respect to electrophoresis? a. DNA is not attracted to either pole but in fact moves by diffusion through the TAE buffer in the agarose. b. DNA can be attracted to either pole depending on the bases it is made of. c. DNA is attracted to the negative pole. d. DNA is attracted to the postive pole
Which of the following statements about DNA probes are false? a. Probes can be labeled with a fluorescent dye to facilitate detection. b. Probes can be labeled with radioactive phosphorus to facilitate detection. c. Probes are typically composed of double stranded pieces of DNA. d. Probes are typically composed of single stranded pieces of DNA.
What causes the DNA to migrate from the wells toward the positive electrode? a) DNA is positively charged too, and so was attracted to the positive electrode b) DNA is negatively charged, and so was attracted to the positive electrode c) DNA is polar and forms hydrogen bonds with the positive electrodes d) DNA is electrically charged, and so cycles through an electric field The ratio of absorption at 260nm to absorption at 280nm is commonly used to assess. (Note: purity of DNA should be the right answer, but a is shown as wrong) a) The concentration of DNA and RNA. b) The purity of protein. c) Whether your DNA or RNA is contaminated Trizol. d) The concentration of protein in your sample. How many different bases are associated with RNA? a) 5 b) 4 c) 3 d) 6
In lane 1, a size standard was loaded, which contained a mix a DNA fragments known to be 1000 bp, 700 bp, 500 bp, 200 bp, and 100 bp. When the gel was run and stained, the following photograph of the gel was taken. Click directly on the band in lane 1 that represents the DNA fragment that is 500 bp in size.
When gel electrophoresis is done correctly, which of these DNA molecules would move the least through the gel? Group of answer choices: 1000 bp molecules 6 Kbp molecules 3 Kbp molecules 2000 bp molecules
Which of the following is true of the gel electrophoresis shown? A) the power source has not been turned on yet. B) the three wells contained the same DNA molecules. C) well #1 had fewer molecules of DNA than well #2 or #3 D) well #3 had more different sized molecules of DNA than well #1 or #2 Which of the following statements is TRUE about the band of molecules labeled X ( close to where it says positive electrode). A) it contains DNA fragments that are shorter than the ones in any of the other bands. B) it contains fewer DNA molecules than any of the other bands from lane 3. C) it contains more negatively charged particles than any other band on the gel. D) it contains fewer genes than any of the other bands on the gel.
The following DNA fragment was sequenced by the Sanger method. The red asterisk indicates a fluorescent label. A sample of the DNA was reacted with DNA polymerase and each of the nucleotide mixtures (in an appropriate buffer) listed below. Dideoxynucleotides (ddNTPs) were addedin relatively small amounts.1. dATP, dTTP, dCTP, dGTP, ddTTP2. dATP, dTTP, dCTP, dGTP, ddGTP3. dATP, dCTP, dGTP, ddTTP4. dATP, dTTP, dCTP, dGTPThe resulting DNA was separated by electrophoresis on an agarose gel, and the fluorescent bands on the gel were located. The band pattern resulting from nucleotide mixture 1 is shown below. Assuming that all mixtures were run on the same gel, what did the remaining lanes of the gel look like?
An individual’s unique set of_______ can be used in DNA profiling. a. DNA sequences c. SNPs b. short tandem repeats d. all of the above

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	help visualize the DNA
	indicate when the DC current is turned on
	serve as a guide to the length of the fragments in the other wells
	indicate when the DNA has completely crossed the gel
	serve as a channel for ethidium bromide