4. ANSWER BELOW QUESTIONS: A. First write the original DNA strand. Indicate where the substitution was by either circling it or writing it in a different color. Then write the mutated DNA sequence with the point mutation (aka substitution) wherever you choose for it to be. Again, circle it or write it in a different color. Do the same for the transcribed mRNA. Repeat the directions for 2 and 3 for this new DNA stand. (i.e., include the mRNA and translated protein of the mutated DNA. Remember your mutated DNA strand should look just like the original DNA strand with one letter changed-either circled or in a different color).   B. Indicate if the mutation resulted in a synonymous substitution or nonsynonymous substitution.   C. If the mutation was nonsynonymous, indicate which type of nonsynonymous substitution it was.   D. Indicate if the results of the mutation likely beneficial, likely harmful or likely neutral.

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter29: Transcription And The Regulation Of Gene Expression
Section: Chapter Questions
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ANSWERS 1-3 were answered but I need 4 and 5 answered please.

Three parts are solved in case of interlinked question as per our company policy. If you want assistance with other parts, please post separately. 

The DNA is made up of deoxyribonucleotide sub units. It is the genetic material.

Step 2

The deoxyribonucleotides consist of deoxyribose sugar, phosphate group and nitrogenous bases. Nitrogenous bases are adenine, guanine, thymine and cytosine. 

Answer 1) The sequence of template strand in 3' to 5' sequence is:

3' TACCCGCCAGCCTACATC 5'

Answer 2) 

The mRNA strand is complementary to the template strand. The RNA polymerase synthesises mRNA. The A, U, G and C are present in mRNA with respect to the T, A, C and G in DNA. 

The mRNA sequence is: 5' AUGGGCGGUCGGAUGUAG 3'

The mRNA sequence in form of codon is: 5' AUG GGC GGU CGG AUG UAG 3' The codon is made up of three nucleotides. 

Answer 3) The mRNA codon chart helps to find the sequence of amino acid from a codon. The start codon initiates translation. Sto codon (UAA, UAG or UGA) terminates translation. The protein sequence is: N- met- gly- gly- arg- met- C 

4. ANSWER BELOW QUESTIONS:

A. First write the original DNA strand. Indicate where the substitution was by either circling it or writing it in a different color. Then write the mutated DNA sequence with the point mutation (aka substitution) wherever you choose for it to be. Again, circle it or write it in a different color. Do the same for the transcribed mRNA. Repeat the directions for 2 and 3 for this new DNA stand. (i.e., include the mRNA and translated protein of the mutated DNA. Remember your mutated DNA strand should look just like the original DNA strand with one letter changed-either circled or in a different color).

 

B. Indicate if the mutation resulted in a synonymous substitution or nonsynonymous substitution.

 

C. If the mutation was nonsynonymous, indicate which type of nonsynonymous substitution it was.

 

D. Indicate if the results of the mutation likely beneficial, likely harmful or likely neutral.

 

5. ANSWER BELOW QUESTIONS:

A. Repeat number 4 (a through d), except do a deletion or insertion mutation, by subtracting or adding a nucleotide from the original DNA sequence from number 1. (Don’t forget to rewrite the original DNA sequence, and mutated DNA strand.)

 

B.

 

C.

 

D. Indicate if the results of the mutation is always beneficial, always harmful or always neutral.

 

 

b. Indicate if the mutation resulted in a synonymous substitution or nonsynonymous
substitution.
C.
If the mutation was nonsynonymous, indicate which type of nonsynonymous
substitution it was.
d. Indicate if the results of the mutation likely beneficial, likely harmful or likely neutral.
5.
a. Repeat number 4 (a through d), except do a deletion or insertion mutation, by
subtracting or adding a nucleotide from the original DNA sequence from number 1.
(Don't forget to rewrite the original DNA sequence, and mutated DNA strand.)
b.
C.
d. Indicate if the results of the mutation is always beneficial, always harmful or always
neutral.
Transcribed Image Text:b. Indicate if the mutation resulted in a synonymous substitution or nonsynonymous substitution. C. If the mutation was nonsynonymous, indicate which type of nonsynonymous substitution it was. d. Indicate if the results of the mutation likely beneficial, likely harmful or likely neutral. 5. a. Repeat number 4 (a through d), except do a deletion or insertion mutation, by subtracting or adding a nucleotide from the original DNA sequence from number 1. (Don't forget to rewrite the original DNA sequence, and mutated DNA strand.) b. C. d. Indicate if the results of the mutation is always beneficial, always harmful or always neutral.
Mutation Worksheet
1. Create a DNA sequence with eighteen nucleotides. Indicate its 3' on the left and 5' on the
right since that's the template strand you will need in the next question to transcribe the
mRNA.
2. Transcribe the DNA sequence above and separate the triplets into codons. Indicate 5' and 3'
in the correct location on the strand. (Don't worry about splicing- assume that the pre-
mRNA is the same as the mature mRNA sequence)
3. Look at the genetic code, and indicate which amino acid is coded for by the codons in the
above mRNA.
4.
a. First write the original DNA strand. Indicate where the substitution was by either circling
it or writing it in a different color. Then write the mutated DNA sequence with the point
mutation (aka substitution) wherever you choose for it to be. Again, circle it or write it in
a different color. Do the same for the transcribed mRNA. Repeat the directions for 2 and
3 for this new DNA stand. (i.e., include the mRNA and translated protein of the mutated
DNA. Remember your mutated DNA strand should look just like the original DNA strand
with one letter changed-either circled or in a different color).
Transcribed Image Text:Mutation Worksheet 1. Create a DNA sequence with eighteen nucleotides. Indicate its 3' on the left and 5' on the right since that's the template strand you will need in the next question to transcribe the mRNA. 2. Transcribe the DNA sequence above and separate the triplets into codons. Indicate 5' and 3' in the correct location on the strand. (Don't worry about splicing- assume that the pre- mRNA is the same as the mature mRNA sequence) 3. Look at the genetic code, and indicate which amino acid is coded for by the codons in the above mRNA. 4. a. First write the original DNA strand. Indicate where the substitution was by either circling it or writing it in a different color. Then write the mutated DNA sequence with the point mutation (aka substitution) wherever you choose for it to be. Again, circle it or write it in a different color. Do the same for the transcribed mRNA. Repeat the directions for 2 and 3 for this new DNA stand. (i.e., include the mRNA and translated protein of the mutated DNA. Remember your mutated DNA strand should look just like the original DNA strand with one letter changed-either circled or in a different color).
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Where is the substitution indicated on the original DNA strand? May you please Write it out and bold the nucleotide.

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