Which among the following statements is correct? Negatively-charged proteins are eluted out first when a cation exchanger is used as the stationary phase for exchange chromatography O Proteins that have higher affinity to the ligands are eluted out last in affinity chromatography OBoth statements are correct O Both statements are incorrect
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- Which among the following statements is correct? Group of answer choices Both statements are incorrect Ion-exchange chromatography is dependent on the charge and size of the target component Both statements are correct Affinity chromatography is dependent on the affinity of the target component to the mobile phaseAn IV bag of NS containing 30 g KCl is being administered at a rate of 80 mL/hr using a macrodrip tubing with a DF of 15 gtts/mL. How many drops per minute is that? Group of answer choices a. 30 gtts/min b. 22 gtts/min c. 10 gtts/min d. 20 gtts/minIf Ca(NO3)2 solution is added to the saturated CaSO4 solution;Solubility product of CaSO4 (KÇ)II. Solubility of CaSO4III. The concentration of Ca + 2 ionWhich of these or which decreases at the same temperature? A) I only B) II only C) III only D) I and II E) II and III
- What is ion-exchange chromatography based on? moving a molecule with a net charge in an electric field high affinity of the antibody for the proteins separation of proteins by size affinity of proteins for specific chemical groups or specific molecules separation of proteins by their net chargeDefine the following terms:a. ligandb. motor proteinc. GTPd. macromolecular crowdinge. excluded volumeGiven these three statement- two of which are truths and one is a lie. Which of the following statements is a lie? Select one: a. Unless a channel of a carrier protein is open, cations remain outside. b. When we experimentally induce a positive charge in the inside of the cell, cations will be forced out. c. Equal concentrations are achieved in the ions involved in the electrochemical gradient. Given these three statements - two of which are truths and one is a lie. Which of the following statements is a lie? Select one: a.The primary active transport prepares the conditions of the membrane gradient by making the extracellular membrane have a less negative charge in the concentration gradient than the intracellular membrane. b. The primary active transport uses ATP while secondary transport helps replenish ATP. c. Both primary and secondary transport moves solutes against their concentration gradients.
- Define the following terms:a. supersecondary structureb. protomerc. phosphoproteind. denaturatione. ion exchange chromatographyDefine the following terms:a. chemiosmotic coupling theoryb. uncouplerc. ionophored. submitochondrial particlee. a,b hexamerSelect all the true statements about ion exchange chromatography Group of answer choices In anion exchange, negatively charged species are retained on the column, whereas positively charged ones go through the column unretained In cation exchange, negatively charged species are retained on the column, whereas positively charged ones go through the column unretained In anion exchange, a protein with a pI of 9.0 will not get retained if the mobile phase has a neutral pH In cation exchange, a protein with a pI of 9.0 will get retained if the pH of the mobile phase is acidic
- Membrane carriers resemble enzymes except for the fact that carriersa. are not proteins.b. do not have binding sites.c. are not selective for particular ligands.d. change conformation when they bind a ligand.e. do not chemically change their ligands.We have isolated a mixture of 2 protein of the same molecular weight but different isoelectric points. The isoelectric points are 7.5 for Protein A and 9.0 for Protein B. What would be the order of elution from a cation exchange column if a buffer of pH 7 is used? Group of answer choices Both proteins will remain in the column. Both proteins will elute simultaneously Protein B followed by Protein A Protein A followed by Protein BDesign an experiment to purify protein X on an anion-exchange column. Protein X has an isoelectric point of 7.0.