Which of the below conditions would result in the lowest level of expression from the lac operon High lactose, low glucose, mutation in lac repressor so that it can bind the operator and lactose at the same time High lactose, high glucose, mutation in sigma 70 protein that increases its affinity for binding the promoter No lactose, deletion of the operator sequence, low glucose High lactose, high glucose, changing the-35 and -10 region of the promoter to the consensus sequend for sigma 70 binding

Which of the below conditions would result in the lowest level of expression from the lac operon High lactose, low glucose, mutation in lac repressor so that it can bind the operator and lactose at the same time High lactose, high glucose, mutation in sigma 70 protein that increases its affinity for binding the promoter No lactose, deletion of the operator sequence, low glucose High lactose, high glucose, changing the-35 and -10 region of the promoter to the consensus sequend for sigma 70 binding

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 9TYU: A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous...

See similar textbooks

Similar questions

A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous transcription of the structural genes (b) no transcription of the structural genes (c) the binding of the repressor to the operator (d) no production of RNA polymerase (e) no difference in the rate of transcription
INTERPRET DATA Develop a simple hypothesis that would explain the behavior of each of the following types of mutants in E. coli. Mutant a: The map position of this mutation is in the trp operon. The mutant cells are constitutive; that is, they produce all the enzymes coded for by the trp operon, even if large amounts of tryptophan are present in the growth medium. Mutant b: The map position of this mutation is in the trp operon. The mutant cells do not produce any enzymes coded for by the trp operon under any conditions. Mutant c: The map position of this mutation is some distance from the trp operon. The mutant cells are constitutive; that is, they produce all the enzymes coded for by the trp operon, even if the growth medium contains large amounts of tryptophan.
Assuming that the level of glucose is low, a mutation in the repressor associated with the lac operon of E. coli which prevents binding of the repressor to lactose should result in_____ Constitutive expression of the lac operon genes ito sagot ko Lack of expression or reduced expression of the lac operon genes under all circumstances Expression of the genes only when lactose is present Expression of the genes only when lactose is absent
High levels of glucose in the environment result in low levels of transcription of the lac operon. The reason for this is ____________________________________________. glucose lowers the levels of the CAP protein, which is necessary to stimulate transcription of the lac operon glucose binds to the Lac Repressor to cause it to bind to the operator site (lac O) glucose raises the levels of cAMP, which binds to the CAP protein to cause transcription repression glucose prevents lactose from binding to the Lac Repressor glucose lowers the levels of cAMP, which is necessary to activate the CAP protein
Which of the following condition(s) is optimal for expression of the lac operon? I. low glucose and formation of CAP-cAMP complex II. high glucose resulting in no cAMP III. no lactose present and a functional repressor IV. presence of lactose binding to a functional repressor
Which of the following describes the lac operon in E. coli when lactose and glucose is present in the culture medium? Select one: a. CAP inactive and RNA polymerase does not completely bind to the promoter of the lac operon and transcription of lactose-metabolizing enzymes decreases. b. CAP is active and RNA polymerase binds completely to the promoter of the lac operon and transcription of lactose-metabolizing enzymes decreases. c. Catabolite activator protein (CAP) inactive and RNA polymerase does not completely bind to the promoter of the lac operon and transcription of lactose-metabolizing enzymes increases. d. CAP is active and RNA polymerase binds completely to the promoter of the lac operon and transcription of lactose-metabolizing enzymes increases.
A mutation in the repressor LacI causes it to lose the ability to bind its effector, allolactose. What will be the effect of this mutation in the presence of allolactose (assume glucose is absent)? a) LacI cannot bind to the lac operator (lacO) and transcription of the lac operon is blockedb) LacI is permanently bound to the lac operator (lacO) and transcription of the lac operon is blocked c) LacI cannot bind to the lac operator (lacO) and transcription of the lac operon increasesd) LacI is permanently bound to the lac operator (lacO) and transcription of the lac operon increases
In the lac operon, the lacZ gene codes for an enzyme called ____________, which catalyze the breakdown of the sugar __________ into monosaccharides. This enzyme can also hydrolyze the sugar analog ____________, which produces blue pigments following its breakdown. Since the lac operon is not always on the bacterial cells, we add the chemical ________ which prevents the lac depressor from binding to the operators, leading to constitutive expression of the operon.
For the lac operon, when glucose is absent and lactose is present ___________ None of the options cAMP-CAP is bound to the operator and the repressor is not bound to the promoter cAMP-CAP is not bound to the promoter and the repressor is bound to the operator cAMP-CAP is bound to the promoter and the repressor is not bound to the operator
An E. coli cell acquires a mutation in the gene that encodes CAP. The mutation affects the DNA-binding domain of CAP and ablates the ability of CAP to bind to the CAP site. Under which condition will the genes of the lac operon be transcribed at high levels? Glucose absent, lactose absent Glucose absent, lactose present Glucose present, lactose absent Glucose present, lactose present Under any of these conditions, the lac operon will not be transcribed at high levels
What are the effects of the following conditions on Lac operon of bacteria? Please do not forget to mention about the role of repressor, activator, RNA polymerase in each case. Glucose is absent and lactose is present Glucose is present and lactose is present Glucose is present and lactose is absent
The figure above shows the structure of lac operon. Which of the following BEST describes what happens in the presence of lactose? Select one: Repressor-lactose complex forms, both the complex and RNA polymerase bind to R. Transcription of S is initiated. Repressor-lactose complex forms and the complex will not bind to R. Transcription of S is initiated. Both repressor and RNA polymerase bind to R. Transcription of S is initiated. Repressor-lactose complex forms and the complex binds to R. Transcription of S is prevented.