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- The formation of deoxyribonucleotide from ribonucleotide requires all the following except: a.Nucleoside diphosphate (NDP) b. Nucleoside triphosphate (NTP) c.Thioredoxin d. Ribonucleotide reductaseWhich of the following enzyme:function pairs is/are correct? a.primase:forms RNA primer b.ligase:join Okazaki fragments c.gyrase:relax supercoils d.DNA POI ll:removes RNA primersand set up a series of four dideoxy reactions (the dideoxy nucleotides are labeled with radioactive P32 ). You then separate the products of the reactions by gel electrophoresis and obtain the following banding pattern: a) Write out the base sequence of the newly synthesized strand from reading the gel (include the 5’ and 3’; end) b) Write out the base sequence of the original fragment you were given (include the 5’ and 3’ end)
- DNA reacts with Biuret through ______________, and shows an absorbance in the visual region at ____________. DNA does not react with Biuret reagent Backbone phosphate groups; 280 nm Backbone phosphate groups; 260 nm Backbone phosphate groups; 540 nmDNA is visualized during agarose gel electrophoresis by ______________ . the fact that DNA fluoresces when illuminated with UV light the binding of a fluorescent dye that is easily detectable using radioactive antibodies that specifically bind to DNA the fact that DNA is blue and can be seen when millions of copies are present in a bandWhat DNA sequence pattern does HindIII enzyme recognize and cleave? Setup the reaction conditions for HindIII enzyme (to digest 1 ug of DNA). Make sure to list all components necessary including the buffers.
- The enzymes mentioned below are used as tools during cloning, DNA sequencing and/or gene therapy. Explain what they are used for. Also mention the actual biological function of the respective enzymes. 1) RNaseHDraw out the following DNA sequence on a white sheet of paper along with itscomplementary strand. Challenge yourself by not using any notes to begin with. Use hexagon and hexagon/pentagon shapes within the nitrogenous bases to depict purines and pyrimidines. Use circles and pentagons to show phosphates and sugars in the backbones. Label the ends (5’ and 3’) and make sure to associate those ends with the correct functional group. Include the proper number of hydrogen bonds between base pairs. 3’ CCAGGTACT 5’Describe the function of the following reagents used in the DNA extraction procedure?a) Proteinase K b) 5M Nacl c) Isopropanol d) 1X TE Buffer
- Which of the following statements is true?a) Exonuclease III removes nucleotide residues from the 3’ ends of a DNA strandb) Bacteriophage lambda exonuclease removes terminal phosphatesc) Alkaline phosphatase removes nucleotides from 5‘endsd) Kinase adds homopolymer tails to the 3’ –OH ends of a linear duplexMake the complementary strand for the following DNA template and label both strands as 5 to 3 or 3 to 5 (P = phosphate in the diagram). Draw an arrow showing the direction of synthesis of the new strand. How many hydrogen bonds are in this double strand of DNA? template: PAGGCTCGOH new strand:Restriction enzymes are used in laboratory manipulations to cut DNA; many of these enzymes can be inactivated by incubating them at 80°C for 30 minutes. Why would this incubation inactivate the enzyme? Why do enzymes not simply operate better at higher temperatures, as other catalysts do?