Which of the following best explains the natural function to the tumor-inducing (Ti) plasmid? It expresses a toxin that causes tumors in insects, making them avoid soil containing Agrobacterium They are used cause tumors in mice, in order to study cancer genetics It is used by Agrobacterium to induce tumors in its plant host, so the plant will make food for the bacteria It is used by geneticists to make transgenic plants

Which of the following best explains the natural function to the tumor-inducing (Ti) plasmid? It expresses a toxin that causes tumors in insects, making them avoid soil containing Agrobacterium They are used cause tumors in mice, in order to study cancer genetics It is used by Agrobacterium to induce tumors in its plant host, so the plant will make food for the bacteria It is used by geneticists to make transgenic plants

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter15: Dna Technology And Genomics

Section: Chapter Questions

Problem 10TYU: DNA molecular with complementary sticky ends associate by (a) covalent bonds (b) hydrogen bonds (c)...

See similar textbooks

Similar questions

DNA molecular with complementary sticky ends associate by (a) covalent bonds (b) hydrogen bonds (c) ionic bonds (d) disulfide bonds (e) phosphodiester linkages
Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner left the foreign genomic DNA that you are planning to clone on the lab bench overnight instead of storing it in the freezer. As a result, it was degraded by nucleases, but still used in the experiment. The plasmid, on the other hand, is fine. What results would you expect from your molecular cloning experiment? There will be no colonies on the bacterial plate. There will be blue colonies only. There will be blue and white colonies. The will be white colonies only.
The following statements are true about common gene cloning procedures except: -DNA plasmids can help move around genes into other cells - Restriction enzymes are very important for cutting up and linking chunks of DNA -We make it so that genes from plants or animals are expressed in bacteria so the products can be harvested - DNA plasmids are chunks of chromosomal DNA used for cloning
A.) Transformation is best described as: Group of answer choices The integration of foreign DNA into a genome The uptake of naked DNA from the environment The transfer of DNA via a bacteriophage Transfer of a plasmid from one organism to another B.) What is the function of the araC gene in the pGLO plasmid? Group of answer choices It encodes the protein that glows under ultraviolet light It allows us to select for the cells that contain the plasmid It prevents the transcription of the green fluorescent protein unless arabinose is added It ensures that the plasmid will be copied and passed on to daughter cells C.) What is the function of the bla gene in the pGLO plasmid? Group of answer choices It encodes the protein that glows under ultraviolet light It allows us to select for the cells that contain the plasmid It prevents the transcription of the green fluorescent protein unless arabinose is added It ensures that the plasmid will be copied and passed on…
Transgenic bacteria can be used to make an alanine rich (GM) plant. Explain how bacteria can be used to produce large amounts as a cheap source of protein. Your explanation will include the role of: Restriction enzymes, plasmids, recombinant DNA, and bacteria.
The Molecular Cell Biology Unit of University X is working on a particular gene segment called ABC20. For a specific kind of experiment, they need to quantify the gene and require a lot of copies of the gene. The initial gel run showed a very faint band of the gene which made the quantification process difficult to proceed. Now propose a method with the help of which they can generate many copies of the gene. Here you need to know that they have the plasmid from where they can get the gene. You do not need to explain the process in detail rather answer in no more than 10 sentences on what can be done about this problem!
A plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for kanamycin resistance (kanR). There is a restriction site inside of the strR gene that you will use to move in your gene of interest. After cutting both your vector and insert you mix them together in a test tube. You then transform your mixture into E. coli and plate your cells on a master plate without any antibiotics. After colonies appear, you pick and re-plate onto a streptomycin containing plate and onto a kanamycin containing plate. What do you expect to grow on the master plate without any antibiotics? What do you expect to grow on the streptomycin plate? What do you expect to grow on the kanamycin plate? How do you determine which colonies contain your gene of interest? As mentioned above, there is a restriction site found within the strR Please draw or write out two different examples of what restriction sites may look like. These should be short…
Cloning vector contains a multiple cloning site (MCS) or polylinker containing multiple unique restriction sites. It also contains markers that allow for rapid selection of colonies with insert-containing plasmids in them. The 1st statement is True and the 2nd statement is False. The 1st statement is False and the 2nd statement is True. Both Staments are True. Both statements are False.
A biotech lab technician is working with a mutated form of a particular bacterial gene and wants to obtain large amounts of plasmid DNA that contains this gene. In this situation, the gene should be in which of the following type of vector (to introduce into E. coli)? Expression vector. Shuttle vector. F plasmid. Cloning vector.
A plasmid vector has two genes in it, a gene for penicillin resistance (penR) and another for chloramphenicol resistance (camR). There is a restriction site inside of the penR gene that you will use to move in your gene of interest. After cutting both your vector and insert you mix them together in a test tube. You then transform your mixture into E. coli and plate your cells on a master plate without any antibiotics. After colonies appear, you pick and re-plate onto a penicillin containing plate and onto a chloramphenicol containing plate. What do you expect to grow on the Master Plate? A. E. coli with the gene of interest in your plasmid vector B. Impossible to tell with the provided information C. Nothing will grow D. E. coli with the original plasmid vector E. E. coli with no plasmid vector
A plasmid vector has two genes in it, a gene for penicillin resistance (penR) and another for chloramphenicol resistance (camR). There is a restriction site inside of the penR gene that you will use to move in your gene of interest. After cutting both your vector and insert you mix them together in a test tube. You then transform your mixture into E. coli and plate your cells on a master plate without any antibiotics. After colonies appear, you pick and re-plate onto a penicillin containing plate and onto a chloramphenicol containing plate. What do you expect to grow on the Chloramphenicol Plate? Select one or more: a.E. coli with no plasmid vector b.Impossible to determine with the provided information c.Nothing will grow d.E. coli with the gene of interest in your plasmid vector e.E. coli with the original plasmid vector
A plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for kanamycin resistance (kanR). There is a restriction site within the strR gene that you are hoping to move your gene of interest into. After cutting both your vector and insert you mix them together in a test tube. You then transform your mixture into E. coli and plate your cells on a master plate without any antibiotics. After colonies appear, you pick and re-plate onto a streptomycin containing plate and onto a kanamycin containing plate. 1. Using those same pieces of short DNA that you created above, show me what one of them may look like if it was cut with a blunt end restriction endonuclease. With the other example, show me what it would look like if it was cut with a sticky end restriction endonuclease. 2. Why do restriction endonucleases exist in bacterial cells? What must they do to protect their own DNA?