Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Question
Please put in correct order
You are cloning a gene from a eukaryotic organism into a plasmid to produce a recombinant protein in bacteria. Put the following steps in the correct order Screen the colonies that contain the right construct Grow bacteria for the expression of the gene you cloned Insert the gene into the plasmid by ligation reaction Plate the transformation reaction Digest the plasmid with the same restriction enzymes Run an agarose gell and isolate the DNA fragments Synthesize CDNA Transform the bacteria with the plasmid you constructed Isolate the mRNA Create restriction sites by PCR P Type here to search
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Transcribed Image Text:You are cloning a gene from a eukaryotic organism into a plasmid to produce a recombinant protein in bacteria. Put the following steps in the correct order Screen the colonies that contain the right construct Grow bacteria for the expression of the gene you cloned Insert the gene into the plasmid by ligation reaction Plate the transformation reaction Digest the plasmid with the same restriction enzymes Run an agarose gell and isolate the DNA fragments Synthesize CDNA Transform the bacteria with the plasmid you constructed Isolate the mRNA Create restriction sites by PCR P Type here to search
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