A piece of human DNA is treated with two common restriction enzymes. The DNA piece in question is 50 Kbp or 50,000 base pairs (bp) long. The numbers of fragments obtaincd with one of the enzymes was estimated by computer analysis to be about 200 fragments, while with the other enzyme the number of recorded fragments was only about 10. You later find that two enzymes had the following palindrome as recognition sequence on DNA. 5'. -GAATTC---- --3' 3'- -СТТАAG- Enzyme-1 5' 3'. --GATC- --CTAG-- Enzyme-2 EXPLAIN quantitatively these results? Which enzymes generated cach fragment number and why?

Human Anatomy & Physiology (11th Edition)
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A piece of human DNA is treated with two common restriction enzymes. The DNA piece in question
is 50 Kbp or 50,000 base pairs (bp) long. The numbers of fragments obtained with one of the enzymes
was estimated by computer analysis to be about 200 fragments, while with the other enzyme the
number of recorded fragments was only about 10.
You later find that two enzymes had the following palindrome as recognition sequence on DNA.
5'
---GAATTC-
-3'
3'-
--СТТААG-
--5
Enzyme-1
5'
-GATC-
-3'
3'
-СТAG--
Enzyme-2
|
EXPLAIN quantitatively these results? Which enzymes generated each fragment number and why?
Transcribed Image Text:A piece of human DNA is treated with two common restriction enzymes. The DNA piece in question is 50 Kbp or 50,000 base pairs (bp) long. The numbers of fragments obtained with one of the enzymes was estimated by computer analysis to be about 200 fragments, while with the other enzyme the number of recorded fragments was only about 10. You later find that two enzymes had the following palindrome as recognition sequence on DNA. 5' ---GAATTC- -3' 3'- --СТТААG- --5 Enzyme-1 5' -GATC- -3' 3' -СТAG-- Enzyme-2 | EXPLAIN quantitatively these results? Which enzymes generated each fragment number and why?
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