From your knowledge about DNA microarray, answer the following: A- How DNA microarray is created? and why it is referred to as “hybridization technology”? B- Why RT-PCR is important in the sample preparation to perform expression microarray experiment? C- Mention the name and the color of the dyes used in expression microarray?
From your knowledge about DNA microarray, answer the following:
A- How DNA microarray is created? and why it is referred to as “hybridization technology”?
B- Why RT-PCR is important in the sample preparation to perform expression microarray experiment?
C- Mention the name and the color of the dyes used in expression microarray?
D- If the expression microarray experiment was done with a normal sample and a suspected sample, after reading the color pattern resulted from the experiment it was recorded that “gene A22” is expressed in the suspected sample. The gene A22 is clinically linked to colon cancer. Answer the following: What is the expected color of the spot on the microarray which represents this gene? What is your interpretation of the suspected sample; is it a cancer sample or not and explain why?
Step by step
Solved in 3 steps
