Which of the following statements about CRISPR-Cas9 is correct? O A. It relies on the natural process of DNA repair. B. Regardless of the DNA target, the sequence of the guide RNA is always the same. C. Cas9 is only involved in recruitment of guide RNA to its target DNA. D. It is not precise enough to make a single base pair edit.

Which of the following statements about CRISPR-Cas9 is correct? O A. It relies on the natural process of DNA repair. B. Regardless of the DNA target, the sequence of the guide RNA is always the same. C. Cas9 is only involved in recruitment of guide RNA to its target DNA. D. It is not precise enough to make a single base pair edit.

Name: Which of the following statements about CRISPR-Cas9 is correct?O A. I
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Description: Which of the following statements about CRISPR-Cas9 is correct?O A. It relies on the natural process of DNA repair.B. Regardless of the DNA target, the sequence of the guide RNA is always the same.C. Cas9 is only involved in recruitment of guide RNA

BIOLOGY:CONCEPTS+APPL.(LOOSELEAF)

10th Edition

ISBN:9781305967359

Author:STARR

Publisher:STARR

Chapter15: Biotechnology

Section: Chapter Questions

Problem 11SA: Put the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA...

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Q. Which of the following statements about CRISPR-Cas9 is correct? A. It relies on the natural process of DNA repair. B. Regardless of the DNA target, the sequence of the guide RNA is always the same. C. Cas9 is only involved in recruitment of guide RNA to its target DNA. D. It is not precise enough to make a single base pair edit.
Attached is a cartoon model of the CRISPR system, with 3 critical components labeled. A. What is the identity of each component? B. What is the function of component a? C. What is the function of component c?
Which of the following is true of reverse transcriptase?a. It is required for the movement of DNA transposons.b. It catalyzes the synthesis of DNA from RNA.c. It is required for the transposition of retrotransposons.d. b and c are correct.
Which of the following is NOT a true statement about CRISPR/Cas9?A. CRISPR/Cas9 is simpler to design than other gene editing technologies.B. CRISPR/Cas9 is derived from a bacterial adaptive immune defense system.C. CRISPR/Cas9 is more efficient than other gene editing technologies.D. CRISPR/Cas9 uses an RNA guide to edit a gene.E. Delivery of CRISPR/Cas9 is easier than delivery of other gene editing technologies.
Which of the following can be a achieved by CRISPR/Cas9-based genome editing tool? A. Knocking out one or more gene B. Activating expression of a gene C. Suppressing expression of a gene D. All of the above
What are the sticky ends of the restriction fragments? Select one: a. The surfaces of sticky ends contain matching base pairs, allowing fragments to splice. b. The surfaces of sticky ends have glue like substance that allow fragments to splice. c. The surfaces of sticky ends contain the exact same nucleotides, allowing fragments to bond. d. The surfaces of sticky ends have velcro like structure, allowing fragments to bond.
What is the principle of the SNP (single nucleotide polymorphisms) in the diagnosis of human diseases? a. PCR product of a gene is different from the expected one b. The size of a recombinant DNA is different from the expected one c. Mutation of a single base in a gene makes the size of a band digested by specific restriction enzymes different from the expected one d. The DNA band detected by Southern blot is different from that by Northern blot
The activity of restriction enzymes may produce fragments with sticky ends. Sticky ends are a) a type of endonucleases. b) dephosphorylated CpG islands. c) unpaired nucleotides. d) double breaks with blunt ends.
Which of the following is a function of a restriction endonuclease? a. It cleaves a DNA molecule at a specific site. b. It cleaves a DNA molecule at random locations. c. It removes specific codons from an mRNA molecule. d. It removes specific exons from an mRNA molecule.
which of the following do researchers not need to use during vector cloning? a. a plasmid containing selectable marker genes such as beta galactosidase or ampicillin resistance genes. b. restriction enzymes. c. DNA polymerase d. a growth medium with carefully selected ingredients that take advantage of selectable markers. e. none of the above.
To synthesize DNA, what does telomerase use as a template? a. It uses the DNA in the 3′ overhang region. b. It uses RNA that is a component of telomerase. c. No template is used. d. Both a and b are correct.
What are the major components of the CRISPR-Cas9 system? What mechanism does it employ to combine DNA? Explain the process of how the CRISP-Cas9 system is able to create recombinant DNA. Relate the idea of gene modification to the fields of vaccines and applied microbiology as well.