Which of the selected phosphate group hydrolysis reactions listed in the table below can NOT be productively coupled to the synthesis of glucose-1-phosphate? Free energy of hydrolysis for certain phosphate bonds Compound kcal/ mol-1 Phosphoenolpyruvate -14.8 1,3-Bisphosphoglycerate -11.8 Creatine phosphate -10.3 ATP (to ADP) -7.3 Glucose 1-phosphate -5 Pyrophosphate -4.6 Glucose 6-phosphate -3.3 Glycerol 3-phosphate -2.2 ATP hydrolysis. Glucose-6-phosphate hydrolysis.
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- Energetic of Fructose-1 ,6-bis P Hydrolysis (Integrates with Chapter 3.) The standard free energy change (G) for hydrolysis of fructose-1. 6-bisphosphate (FBP) to fructose-S-phosphate (F-6-P) and P: is -16.7 KJ/mol: FBP + H2O fructose-6-P + Pi The standard free energy change (G) for ATP hydrolysis is -30.5 KJ/mol: ATP + H2O ADP + Pj What is the standard free energy change for the phosphofructokinase reaction: ATP + fructose-6-P ADP + FBP b. What is the equilibrium constant for this reaction? c. Assuming the intracellular concentrations of [ATP] and (ADP] are maintained constant at 4 mM and 1.6 mM, respectively, in a rat liver cell, what will be the ratio of [FBP]/[fructose-6-P] when the phosphofructokinase reaction reaches equilibrium?The Reactions and Meehanisms of the Leloir Pathway Write the reactions that permit galactose to be utilized in glycolysis. Write a suitable mechanism, tor one of these reactions.Comparing Glycolysis Entry Points for Sucrose Sucrose can enter glycolysis by either of two routes: Sucrose phosphorylase: Sucrose + Pi fructose + glucose-1-phosphate Invertase: Sucrose + H20 fructose + glucose Would either of these reactions offer Jin advantage over the other In the preparation of hexoses fur entry into glycolysis?
- The oxidation of 1 mol of glucose supplies enough metabolic energy to form 36 mol of ATP. Oxidation of 1 mol of a typical dietary fat like tristearin (C57H116O6) yields enough energy toform 458 mol of ATP. How many molecules of ATP can form per gram of (a) glucose; (b) tristearin?Consider the following list of phosphorylated compounds with their free energy changes of phosphate hydrolysis: Glucose-1-phosphate (-5.0 kcal/mol), PEP (-14.8 kcal/mole), 1,3-bisphosphoglycerate (-11.8 kcal/mole) and Glucose-6-Phosphate (-3.3 kcal/mol). Given that the free energy change of ATP hydrolysis is -7.3 kcal/mole, which of these molecules be directly synthesized by the transfer of a phospho- group from ATP? 1,3-bisphosphoglycerate Glucose-6-phosphate All of those phosphorylated compounds. PEP Glucose-1-phosphateThe glycolysis reaction map indicates that is also a substrate for the pentose phosphate pathway (PPP), while product of the PPP and an intermediate for glycolysis. A. \beta-D-Glucose-6-phosphate; glyceraldehyde-3-phosphate B. \alpha - D- Glucose-6-phosphate; glyceraldehyde-3- phosphate C. \alpha-D-Glucose-6-phosphate; glycerate D. \alpha-D-Glucose-6-phosphate; glycerate-3-phosphate is a
- What is the ∆G naught' for a phosphoryl transfer from ATP to glycerol? The hydrolysis of α-glycerophosphate to glycerol and inorganic phosphate has a ∆G naught' of -8.37 kj/mol. ATP + H2O <--> ADP + Pi ∆G naught' = -30.5 kj/mol What is the efficiency of this reaction (i.e. what % of the available energy remains in the system after the reaction)? Express your answer as a % of the total amount of energy available in the system. Where does the rest of the energy go?How many ATP may be produced from 1 mole of the following compounds on complete oxidation using the glycerol-3-phosphate shuttle? COMPOUNDSa.) Mannoseb.) Dihydroxyacetone phosphatec.) Citrated.) Malatee.) SuccinateWhich of the following are examples of structural isomers formed during metabolsim? Select one: a. 3-phosphoglycerate to 2-phosphoglycerate b. Dihydroxyacetone and glyceraldehyde-3-phosphate c. glucose-6-phosphate converted to fructose-6-phosphate d. citrate converted to isocitrate e. All of these are examples of structural isomers.
- If 32P-labeled ATP is provides for the reactions of glycosis, which of the following would be TRUE about 1.3 biphosphoglycerate? A. 50% of 1,3-bis phosphoglycerate would contain 32P B. 100% of 1,3-bis phosphoglycerate would contain 32P on carbon 1 C. 100% of 1,3-bis phosphoglycerate would contain 32 P on carbon 3 D. 50% of 1,3-bis phosphoglycerate would contain 32P on carbon 3 E. 50% of 1,3-bis phosphoglycerate would contain 32P on carbon 1Determine the number of carbon atoms present and the the total number of phosphate groups present in each of these following intermediates of glycolysis: a. Glyceraldehyde 3-phosphate b. 3-PhosphoglycerateThe radiolabel shown on the image in glycogen is used for conversion to ribose 5-phosphate while NADPH is generated. Where would this radiolabel end up? SHOW WORK TO TRACK THE RADIOLABEL Lost as CO2 in the 6-phosphogluconate dehydrogenase reaction C1 (aldehyde function group) of ribose 5-phosphate C2 (next to aldehyde functional group) of ribose 5-phosphate C3 of ribose 5-phosphate C4 of ribose 5-phosphate C5 of ribose 5-phosphate