Which one of the following pairs of compounds are joined together to conserve carbon in the glyoxylate cycle? O Acetyl-CoA + Glyoxylate O Oxaloacetate + Malate CO2+ CO2 Pyruvate + CO2 Ribulose-1,5-Bisphosphate + CO2
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Bacteria and plants subsist on acetate yielding acetyl CoA. They use a metabolic pathway absent in most other organisms that converts 2-Carbon acetyl units into 4-carbon succinate for energy production and biosyntheses.
This Glyoxylate cycle, bypasses the two decarboxylation processes of the citric acid cycle.
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- Which of the following citric acid cycle enzymes catalyzes substrate-level phosphorylation? A) isocitrate dehydrogenase B) α-ketoglutarate dehydrogenase C) succinyl CoA synthetase D) succinate dehydrogenase E) malate dehydrogenaseIn the first step of the TCA cycle the acetyl group of acetyl-CoA is transferred to oxaloacetate to produce citrate. Where will the carbonyl carbon of that acetyl group be after the first CO2 is expelled? Group of answer choices The first carbon of α-ketoglutarate The last carbon of α-ketoglutarate The fourth carbon of α-ketoglutarate The third carbon of α-ketoglutaratealpha-ketoglutarate dehydrogenase will do which of the following: Select one: a. Pyruvate is decarboxylated to become acetyl-CoA producing NADH and Carbon dioxide b. Succinate is oxidized to become fumarate forming FADH2 c. Succinyl-CoA becomes Succinate and forms one ATP molecule and Coenzyme A-SH d. Oxaloacetate combines with the acetyl from acetyl-CoA to produce Citric acid(citrate) e. Malate is oxidized to become oxaloacetate forming NADH f. Isocitrate and then decarboxylated and oxidized to produce alpha-ketoglutarate, Carbon dioxide and NADH g. Citrate is rearranged to become Isocitrate h. Fumarate is combined with water to become Malate i. alpha-ketoglutarate is oxidized and decarboxylated to produce Succinyl-CoA, Carbon dioxide and NADH
- Which of the following is NOT a component of the pyruvate dehydrogenase complex? a. dihydrolipoyl dehydrogenase b. dihydrolipoyl transacetylase c. pyruvate dehydrogenase d. dihydrolipoyl catalaseWhich of the following is a negative regulator of the pyruvate dehydrogenase complex (PDC)? 1. acetyl CoA 2. ADP 3. pyruvate 4. citrateIdentify the Krebs cycle enzyme that consumes a 3-carbon substrate molecule, producing a 2- carbon product molecule (attached to acetyl-CoA), along with NADH, and one molecule of CO2. succinyl CoA synthetase a-ketoglutarate dehydrogenase pyruvate dehydrogenase isocitrate dehydrogenase malate dehydrogenase
- Each of the following citric acid cycle enzymes catalyzes electron transfer to NADH/FADH2 except A) isocitrate dehydrogenase. B) α-ketoglutarate dehydrogenase. C) succinyl CoA synthetase. D) succinate dehydrogenase. E) malate dehydrogenase.Which of the following statements is/are TRUE for the Krebs' cycle? Reaction 1: condensation of acetyl-CoA and oxaloacetate; produces H20. Reaction 3: oxidation of isocitrate to a-ketoglutarate; produces NADH and CO2. Reaction 6: oxidation of succinate to fumarate; produces FADH2 and CO2 Reaction 5: hydrolysis of succinyl-CoA to succinate; produces ATP.If 14CO2 (radioactive carbon) were incorporated into the TCA cycle via the Pyruvate Carboxylase reaction which of the following molecules would contain radioactive carbon? a. Oxaloacetate b. Citric Acid c. Isocitrate d. Alpha-ketoglutarate e. Succinyl-CoA f .Succinate g. Fumarate h. Malate
- The reaction of acetyl-CoA and oxaloacetate in the TCA cycle forms a. 5-carbon α-ketoglutarate b. 4-carbon Succinyl-CoA c. 6-carbon citrate d. 6-carbon α-ketoglutarate e. 4-carbon citrateWhich of the following is true regarding the oxidation of 1 molecule of palmitate to 8 molecules of acetyl CoA by the beta-oxidation pathway? 7 molecules of FADH2 are formed None of the above 8 molecules of FADH2 are formed 8 molecules of NADH are formed BOTH 8 molecules of NADH are formed AND 8 molecules of FADH2 are formedWhich of the following is most likely to cause accumulation of acetyl CoA? Group of answer choices There is not enough oxaloacetate present to run the citric acid cycle Increase activity of pyruvate dehydrogenase kinase Increased fatty acid synthesis Increased citrate synthase activity When fluoroacetate (an analog of acetate) is added to the mitochondria, it causes build up of fluorocitrate. Which of the following is most likely to be true? Group of answer choices Fluoroacetate cannot be converted to fluoroacetyl CoA Fluoroacetyl CoA cannot be combined with oxaloacetate to enter the citric acid cycle Addition of fluoroacetate will cause an increase in isocitrate Aconitase cannot use fluorocitrate as a substrate