Which spectrophotometer mode should you use to calibrate the instrument and which mode should you use to read your samples? Transmittance for both Absorbance for both Absorbance to calibrate and transmittance to read samples Transmittance to calibrate and absorbance to read samples Flip a coin to pick a mode each time.

Spectrophotometer is an optical instrument that is used to measure the light intensity relative to…

Answered: Which spectrophotometer mode should you…

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter19: Biomedical Laboratory Instrumentation

Section: Chapter Questions

Problem 10Q

See similar textbooks

Similar questions

Which of the following is/are source/s of error in performing a spectrophotometric method? Answer all that apply. Sample is turbid when the absorbance is read. The absorbances were read at maximum absorption. Standard solutions are prepared accurately. The cuvettes have fingerprints.
Here are the materials and method for the basic spectrophotometer experiment Materials:1. Paper template2. Scissor3. Light source (i.e. torchlight, portable light)4. Paperboard (black colour)5. Unused compact disc (CD)6. Camera detector (i.e. webcam, laptop, mobile phone) Methods: 1. Make a foldable paper spectrophotometer using the paper template and followthe instruction as in https://publiclab.org/sites/default/files/8.5x11minispec3.8.pdf2. Then, mounted the paper spectrophotometer to the camera detector.3. Download any freely available spectral analyzer.4. Test and observe the wavelength with and without the presence of a light source.5. Finally, observe the wavelength with a different transparent colour sheets (you canchoose any colour). Troubleshooting/ problem(s) encountered for basic spectrophotometer experiment.
Assume your field of view under a 3X objective is 6mm across. What is the field of view in mm under a 20X objective? Show clear work with units for credit. Field of view 20X objective? ___mm What is the field of view of this 20X objective in micrometers? If you observe a specimen under the 20X objective and the specimen spans one-quarter of the field of view distance, how long is the specimen in micrometers?
A student using a microscope measured the low power field diameter to be 7.1mm. The student then switched from the low power (4x) objective lens to the medium-power (10x) objective lens. Assuming a 10 x ocular (eyepiece) lens, the medium power field diameter is ____ um um is micrometers
Your spectrophotometer maxes out at an absorbance reading of 2. If you get a reading of 2, what could you do to your sample to get an accurate reading.
What is the difference between total magnification and objective magnification? You are looking at an organism on the 20x objective What is the total magnification? If the organism takes up 62 units on the ocular micrometer, how big is the organism is micrometers? Show your work.
If a researcher forgot to wipe the outside a sample cuvette to make it free of drops, fingerprints, and dust prior to reading it in the spectrophotometer, how might the absorbance value of the sample be affected? (Higher, Lower, No Change) Explain
Using the scanning (4x) objective and the metric ruler, record the number of millimeters you see along with the letter “e.” Your value: 2 millimeters Convert the figure you attained to micrometers (1 millimeter = 1,000 micrometers). This is the diameter of the field of view for the low-power objective (LPD). The field of view is the circular field you see when you look through the oculars. The field of view changes at different magnifications. Your value (LPD): 2,000 micrometers -please help me with the problem in the picture.
What is the working electrode used for polarography technique? a. Platinum b. Silver c. Gold d. Dropping Mercury Electrode (DME)
how often do we have to clean and caliberate a spirometer based on national and international guidelines
If you observe a specimen under this 20X objective and the specimen spans one-quarter of the field of view distance, how long is the specimen in micrometers?
the figure shows the result of the spectral analyzer for paper spectrophotometer without any transparent paper and with transparent yellow color paper. observe and discuss the intensity and the wavelength for both results.

SEE MORE QUESTIONS

Recommended textbooks for you

Biomedical Instrumentation Systems

Chemistry

ISBN:

9781133478294

Author:

Chatterjee

Publisher:

Cengage

Comprehensive Medical Assisting: Administrative a…

Nursing

ISBN:

9781305964792

Author:

Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:

Cengage Learning

Curren'S Math For Meds: Dosages & Sol

Nursing

ISBN:

9781305143531

Author:

CURREN

Publisher:

Cengage

Biomedical Instrumentation Systems

Chemistry

ISBN:

9781133478294

Author:

Chatterjee

Publisher:

Cengage

Comprehensive Medical Assisting: Administrative a…

Nursing

ISBN:

9781305964792

Author:

Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:

Cengage Learning

Curren'S Math For Meds: Dosages & Sol

Nursing

ISBN:

9781305143531

Author:

CURREN

Publisher:

Cengage

Principles Of Radiographic Imaging: An Art And A …

Health & Nutrition

ISBN:

9781337711067

Author:

Richard R. Carlton, Arlene M. Adler, Vesna Balac

Publisher:

Cengage Learning

SEE MORE TEXTBOOKS

		Transmittance for both
		Absorbance for both
		Absorbance to calibrate and transmittance to read samples
		Transmittance to calibrate and absorbance to read samples
		Flip a coin to pick a mode each time.