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why do We need to Wait for the iron Agar to set and then Add another thin layer of 45°C Iron Agar?
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- Why does motility media contain a lower amount of agar than ordinary agar?If cultures are suffering severely due to alkaloid production and accumulation in the medium then, how can i overcome this problem?What is the major difference between Thayer-Martin and chocolate agar? When would you use Thayer-Martin rather than chocolate agar?
- Why does turbidity in a cultured media losses its reliability at high cell concentrations?Can culture media be sterilized in the hot air sterilizer? Why or why not?What is the advantage of the plating method over an electronic cell counting method in counting cells? Why does turbidity lose reliability at high cell concentrations when the culture reaches the stationary phase?
- In an experiment, the bacteria were placed in dropper bottles containing glycerol as a carbonsource. Why did the culture have to be incubated for an hour in the new media?During media preparation, you observed that the agar medium did not solidify after sterilization even after incubating it for 1 hour at room temperature. What could be the reason behind this?Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Why are petri dishes incubated in an inverted position? Of what advantage is the using a solid and a liquid medium?
- The agar turns pink because the enzyme produces blank which causes a ph change to blank?Aside from Paraffin, what is your recommended infiltrating medium for routine purposes? why?If my tissue cultures are suffering severely due to alkaloid production and accumulation in the medium. In that case, how can I overcome this problem?