Why doesn't the CRISPR system cut the bacterium's own DNA, at the point in its genome where it carries the guide sequence, which came from the original invading bacteriophage?
Q: When would it be beneficial to use a eukaryotic host formolecular cloning?
A: The techniques for gene manipulation, cloning, and expression were first developed in bacteria.…
Q: Suppose that one species of bacteria obtained one gene encoding a restriction endonuclease by…
A: The origin of new genes in an organism is essential for their evolution. These new genes are…
Q: How might a bacteriophage be used to treat bacterial infections in humans?
A: INTRODUCTION Bacteriophages These are the viruses that kills bacteria.
Q: When a virus mistakenly picks up a segment of host bacterial DNA, packages it into a viral particle,…
A: There are many methods of DNA transfer in bacteria such as transduction, conjugation and…
Q: Hershey and Chase used radioactive components to tag protein and DNA before infecting bacteriophage.…
A: Hershey and Chase performed various experiments to confirm which one is a genetic material either…
Q: If a mutagen causes bases to be removed from nucleotides withinDNA, what repair system would fix…
A: DNA repair is the process in which certain specialized enzymes within the cell known as DNA repair…
Q: Why must the gene be inserted into a vector for it to be cloned?
A: Cloning is the process by which a foreign DNA is inserted into the host cell for the purpose of…
Q: Why is the CRISPR system considered a prokaryotic “immunesystem”?
A: Bacteria and archaea produce restriction endonucleases to destroy foreign particles or incoming…
Q: You have discovered a new species of archaea from a hot spring in Yellowstone National Park. After…
A: In prokaryotes, the genome consists of a single DNA molecule. These genomes are much longer than the…
Q: How is Ti plasmid of Agrobacterium tumefaciens modified to convert it into a cloning vector?
A: Agrobacterium tumefaciens is a well-studied species of the gram-negative genus of bacteria, known as…
Q: When an E. coli donor cell duplicates a strand of plasmid DNA, and passes this DNA strand to a…
A: Genetic recombination is the process of exchange of genetic material between two organisms, cells,…
Q: Under most practical circumstances, Crispr-cas9 technology can edit a eukaryote's genome (to replace…
A: Correct response to this particular question would be, 80%.
Q: Why must genetically altered bacteria be grown in a carefully controlled environment?
A: Introduction: The living organisms whose genetic material has been falsely controlled in a lab…
Q: How can the CRISPR bacterial immune system be used to specifically and accurately edit genomes in…
A: CRISPR is a family of DNA sequences found in the genomes of prokaryotic organisms such as bacteria…
Q: Which molecule is responsible for producing the double-stranded breaks that initiate CRISPR genome…
A: The biochemical molecule that is built up with two polynucleotide chains is called Deoxyribonucleic…
Q: frederic griffith used the word transformation to describe the changes in bacteria that he observed.…
A: Frederic Griffith was a British bacteriologist whose experiments proved the existence of a…
Q: What is the benefit of using a strain of Lactobacillus bacterium that contains the CRISPR locus in…
A: CRISPR is a group of DNA sequences present in the genomes of archaea and bacteria, which are…
Q: How is a gene inserted into a plasmid cloning vector?
A: Apart from cell's chromosomal DNA, a double-stranded circular DNA molecule is present in yeast,…
Q: What does the acronym CRISPR stand for?
A: CRISPR is a technique used in editing of base pair of genes. It is important tool in genetic…
Q: When a bacteriophage accidentally incorporates a fragment of host DNA in its capsid and delivers it…
A: Bacteriophages bind to various receptors on the surface of bacteria, such as lipopolysaccharides,…
Q: How has the CRISPR editing technology been appliedto targeting virus-infected eukaryotic cells?
A: The short form CRISPR refers to the Clustered Regularly Interspaced Short Palindromic Sequences.…
Q: Bacteriophage lambda (λ) consists primarily of a head, which contains the genomic DNA, and a tail…
A: Answer: Bacteriophage are the bacteria infecting viruses, which are able to infect bacteria. These…
Q: What is bacteriophage ?
A: Viruses are acellular in nature. They contain a genetic material made up of DNA or RNA which is…
Q: Why is Agrobacterium mediated genetic transformation described as Natural Genetic engineering in…
A: Agrobacterium is a gram-negative bacteria that is responsible for causing tumor in plants by…
Q: Why do bacteria make restriction endonucleases? What is it about the endonucleases that prevents…
A: Restriction enzymes cleave DNA at or close unique recognizing sequences known as restriction sites,…
Q: Why do eukaryotic cells do not contain restriction enzymes?
A: Restriction enzymes are used extensively in molecular biology to cut DNA. One of the unique features…
Q: What normal role do restriction enzymes play in bacteria? How do bacteria protect their own DNA from…
A: A restriction enzyme is a protein that recognizes a particular DNA sequence and cuts the DNA at a…
Q: what is the function of restriction enzymes in digesting products in PCR
A: Restriction enzymes are also known as molecular scissors because they involves in digestion of…
Q: True or False: Restriction enzymes normally prevent viral DNA or RNA entry in bacteria.
A: A bacterium utilizes a restriction protein to defend against bacterial infections called…
Q: Which of the following is required to make complementary DNA (CDNA) from RNA? reverse transcriptase…
A: The central dogma in cell biology is DNA -> RNA -> Protein. The first process is the…
Q: Differentiate between TWO of the following pairs: Genetic and a restriction map Southern and…
A: The mapping of DNA refers to the identification of the location of particular genes. Genetic map and…
Q: Explain The Discovery of CRISPR ?
A: The common technology that can be used to edit genes is refers as the CRISPR. In other words you…
Q: al RNA used to identify a new b
A: RNA stands for the ribonucleic acids. It is a molecules that is similar to DNA as it is also act as…
Q: What characteristics of plasmids and bacteriophages make themgood cloning vectors?
A: Plasmids are small extrachromosomal DNA molecules that are separated from the chromosomal DNA and…
Q: Would it be appropriate to use DNA probes such as VNTR in DNA fingerprinting of an bacteriophage?
A: Any group of Viruses that attach to the bacteria and infect the bacteria is known as bacteriophage.…
Q: Why do restriction endonucleases not hydrolyze DNA from the organism that produces it?
A: Endonucleases are the enzymes, which help to cleave the DNA from inside that is in between the…
Q: In the bacterial transformation experiment, what is the primary purpose of using an…
A: Transformation experiment was given by Griffith. In bacteria, DNA can be transferred using…
Q: When performing phage typing, how would one know whether that bacterium is killed by any of the…
A: Phage typing is a method in which interaction between phage and bacteria is analyzed to detect the…
Q: All of the following enzymes would be used by a bacterium to copy its genome EXCEPT A. Helicase B.…
A: RNA replicase Reichetal showed the RNA synthesis even in the presence of actinomycin-D(that…
Q: What is gene cloning? What is bacterial transformation? What is the difference between the two…
A: Recombinant DNA (rDNA) refers to DNA molecules from two different or distant species that are put…
Q: Why do researchers believe they can use CRISPR/Cas totreat disease?
A: Introduction CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats. This is…
Q: How many tailed bacteriophages are there, and where are they located?
A: According to the question, we have to mention the number of the tailed bacteriophages and where they…
Q: Why bacteriophage is called t4?
A: A bacteriophage is a virus that infects and replicates within bacteria. It injects the genetic…
Q: plasmid cloning vector
A: The term vector refers to the DNA molecules that act as transporting vehicle which carries target…
Q: Which of the following mutagens results in the deamination of nitrogenous bases? O nitrous acid base…
A: The amino bases adenine and cytosine lose one amino group when they are oxidised. As a result, in…
Q: The most usefulplasmids contain a _______, which is a short, syntheticDNA sequence that contains a…
A: Plasmid fingerprinting is performed in many organisms like Escherichia coli, Salmonella,…
Q: Based on the knowledge you gained from the cloning module, which of the bands in the figure is…
A: Cloning modules allows you to have more control over which parts of the original module are…
Q: Which E. coli enzyme is the main replicative polymerase in bacteria that synthesizes the genome?
A: Replication is the process by which new DNA strands are duplicated from the existing DNA strands. It…
Q: a bacteriophage has been isolated from the growth chambers that has evolved the capability to the…
A: In the modern age of technological revolution, various different techniques of genetic engineering…
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- A researcher sequenced a portion of a bacterial gene and obtainedthe following sequence, beginning with the start codon, which isunderlined:ATG CCG GAT TAC CCG GTC CCA AAC AAA ATG ATCGGC CGC CGA ATC TAT CCCThe bacterial strain that contained this gene has been maintainedin the laboratory and grown serially for many generations. Recently,another person working in the laboratory isolated DNA from thebacterial strain and sequenced the same region. The followingresults were obtained:ATG CCG GAT TAT CCG GTC CCA AAT AAA ATG ATCGGC CGC CGA ATC TAC CCCExplain why the differences in the sequences may have occurred.Which E. coli enzyme is the main replicative polymerase in bacteria that synthesizes the genome?HOW Restriction Enzymes Cutthe Genome at Specific Sites?
- Why must the gene be inserted into a vector for it to be cloned?Would it be appropriate to use DNA probes such as VNTR in DNA fingerprinting of an bacteriophage?If the GAATTC palindrome is repeated four times on the same piece of linear DNA, and the restriction enzyme that recognizes that base sequence is present and digests the DNA, how many DNA fragments will be produced?