High-copy- number vector Gene Gene inserted into vector 00 Multiple copies of the gene produced FIGURE 9.4 Cloning into a High-Copy-Number Vector When a gene is inserted into a high-copy-number vector, multiple copies of that gene will be present in a single cell, resulting in the synthesis of many molecules of the encoded protein.

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter28: Dna Metabolism: Replication, Recombination, And Repair
Section: Chapter Questions
Problem 9P: Heteroduplex DNA Formation in Recombination From the information in Figures 28.17 and 28.18, diagram...
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Why must the gene be inserted into a vector for it to be cloned?

High-copy-
number vector
Gene
Gene inserted
into vector
00
Multiple copies of
the gene produced
FIGURE 9.4 Cloning into a High-Copy-Number Vector When a
gene is inserted into a high-copy-number vector, multiple copies of
that gene will be present in a single cell, resulting in the synthesis of
many molecules of the encoded protein.
Transcribed Image Text:High-copy- number vector Gene Gene inserted into vector 00 Multiple copies of the gene produced FIGURE 9.4 Cloning into a High-Copy-Number Vector When a gene is inserted into a high-copy-number vector, multiple copies of that gene will be present in a single cell, resulting in the synthesis of many molecules of the encoded protein.
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