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- Starting with 10 bacterial cells per milliliter in a sufficient amount of complete culture medium with a 1-hour lag phase and a 30-minute generation time, how many cells will there be in a liter of medium at the end of 2 hours? At the end of 7 hours? Show your solution.How would you prepare a 10-7dilution from a culture? Each of your dilution tubes cannot hold more than 1mL.Given a log phase bacterial culture with 1 x 10^6 cells per ml and a generation time of 30 minutes how long does it take the culture to reach a density of 6.4 x 10^7 cells per ml?
- If you have a culture with 1 x 106 cells per ml, how could you use serial dilutions to obtain a suspension with 5 x 102 cells per ml? Show your answer using a serial dilution scheme or diagramYou place 1 ml of a bacterial broth culture into bottle A containing 99 ml of sterile water and mix. You take 1 ml from bottle A and transfer it to bottle B, which contains 99 ml of sterile water. What is the dilution of the bacterial culture in bottle B?On agar plate does each discrete colony represent the growth of one cell? Explain your answer. Why can a single colony on a plate be used to start a pure culture?
- How would you inoculate a plate to get a 1:10 dilution? A 1:100 dilution?Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL bacterial concentration. show solutionYou aseptically transfer 1ml of your original liquid culture into 999 ml of sterile water. What is the dilution factor?
- based on the attached photo, compute the cfu/ml of 42 colonies at plate 10^5. show the complete solution and provide explanation.What would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x 10-2? What would the initial concentration of a bacteria culture be if the final concentration was 3.7 x 102 cells/ml and the total dilution was 4.6 x 10-4?what is the physical characteristics of this streak plate? Look at a single colony on a streak plate and look for special physical characteristics such as: motility, possible presence of endospores and/or capsule, culture color?