Saved Label the following parts of this typical light microscope. Mechanical stage Light source Ocular lens Fine focus Primo Star Coarse focus Mechanical stage Revolving nosepieces Conderser Lamp voltage control Lamp voltage control Objective lens Objective lens Conderser Ocular lens Diaphragm Revolving nosepieces Fine focus Coarse focus Diaphragm Light source
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- What is the highest magnification of the following microscopes?Bright fieldDark fieldPhase-contrastFluorescenceConfocalScanning EMTransmission EMScanned-ProbeFor what purpose would you adjust each of the following microscopecomponents during a microscopy exercise?a. Iris diaphragm:b. Coarse-adjustment knob:c. Fine-adjustment knob:d. Condenser:e. Mechanical stage control:Differentiate between the limit of resolution of the typical light microscope and that of the unaided human eyes the relationship between the depth of field and magnification is that When magnification increases, the depth of field decreases. Name two procedures (steps) you should do to achieve the maximum resolution when using the oil immersion lens State the relationship between the working distance of an objective lens and its magnification power “The coarse focus knob can be used to adjust the focus when using any of the objective lenses”. Is this statement true or false? If it is false, please correct it. When using the oil immersion lens but you cannot focus, what should you should not do? And what you should do? What does this statement mean: “Your microscope is parfocal”? What is the significance of condenser lens? Multiple choice question: The most useful adjustment for increasing image contrast in low power magnification is _______ Closing down the diaphragm Closing one…
- As with standard phase contrast microscopy,differential-interference contrast microscopy depends on differences in the ___________________ of a speciment to create high-contrast images. a. refractive index b. light absorption c. natural fluorescence d. walls and coverings e. natural pigmentationidentify the parts and functions of a microscope. Provide your answers on the spaces provided below. Fine Focus ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Coarse Focus ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Stage Clip ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Rack Stop…Complete the following table by calculating the total magnification of the different objectives of the microscope with the given eyepieces. OBJECTIVES OCULAR Designation Magnification Focal Length Numerical Aperture (NA) Final magnification with: 10x 15x 25x Scanner 4x 46mm 0.10 LPO 10x 16mm 0.25 HPO 40x 4mm 0.55 OIO 100x 1.8mm 1.30
- Compare bright-field, dark-field, phase-contrast, confocal, andfluorescence microscopy as to field appearance, specimen appearance,light source, and uses.Why is CSLM able to view different layers in a thick preparationwhile bright-field microscopy cannot?A microscope that remains in focus regardless of whichobjective lens is rotated into place is __________.
- What major advantage does phase-contrast microscopy haveover staining?How does differential interference contrastmicroscopy differ from bright-field microscopy?How will the following affect resolution during microscopy? I) Closing or opening the diaphragm II) Raising or lowering the condenser III) Increasing or reducing the light intensity