Why is phospholipase c is the target on this western blot explain in detail
Q: In the lac operon system, do the inducers act at the transcription or translation level? Explain why…
A: The lactose operon (lac operon) consists of a regulatory gene (i-gene) and three structural genes -…
Q: The following diagram represents one of the Christmas-tree-like structures shown in Figure On the…
A: DNA molecule is composed of two polynucleotide chains and coil in a double helix. The molecule…
Q: In a single-locus homology-directed repair (HDR), CRISPR/Cas9 machinery makes breaks in the dsDNA at…
A: 2
Q: Why is a host-cell tRNA needed for reverse transcription?
A: Reverse transcription is a process used by retroviruses(RNA viruses) which encodes its RNA genome…
Q: What is the advantage to transferring proteins bands from a gel to nitrocellulose during the western…
A: The western blot is a broadly utilized scientific procedure in molecular biology and immunogenetics…
Q: What would happen if the operator sequence of the trp operon contained a mutation that prevented the…
A: Trp Operon is an operon that codes for the components for the production of Tryptophan, it consists…
Q: he bands indicated by arrow "A" (to the left of the western blot) most likely represent: A.…
A: The bands indicated by arrow "A" (to the left of the western blot) most likely represent:-
Q: Name a special enzyme used by a "copy and paste" transposable element.
A: Transposable elements Transposable elements are sequence found in DNA that have ability to move…
Q: Why is the CRISPR system considered a prokaryotic “immunesystem”?
A: Bacteria and archaea produce restriction endonucleases to destroy foreign particles or incoming…
Q: Why trp operon has a higher level of expression than live operon in bacteria grown on nutrient-poor…
A: Operon is a genetic regulatory system that is found in bacteria and viruses. This system controls…
Q: The trp operon is a repressible operon system. What does this mean?
A: Trp refers to tryptophan. The operon is the functional unit of Deoxyribonucleic acid ( DNA) having…
Q: What is Western blotting ?
A: Blotting refers to a technique by which a biomolecule is transferred from one medium to another…
Q: Northern blotting depends on the phenomenon of the binding of aprobe to mRNA. In this technique,…
A: Northern Blotting is a blotting technique used to detect the presence of a specific ribonucleic acid…
Q: What is west Blotting
A: Blotting is a technique used in molecular biology to identify whether a given sample is DNA or RNA…
Q: Distinguish between a Northern, Southern and Western blot
A: Southern blotting is a blotting process for detecting particular DNA sequences, Northern blotting is…
Q: The first person to receive gene therapy was a young girl with adenosine desminase(ADA) deficiency.…
A: Gene treatment endeavors to treat cancer or boost the body's capacity to combat illness by…
Q: what is the difference between western blot and sds page
A: A western blot is a laboratory method used to detect specific protein molecules from among a mixture…
Q: What is gene therapy? Illustrate using the example of adenosine deaminase (ADA) deficiency.
A: Genetic engineering is a process through which the desired gene of interest is introduced into the…
Q: If serendipity means an unintended but beneficial result, how would you relate this term to describe…
A: The literal meaning of serendipity is the finding of a phenomenon that is useful, beneficial, and…
Q: Give one condition for the denaturation step employed in blotting.
A: one condition for the denaturation step employed in blotting
Q: Why is it necessary to boil the probe before you use it in your southern blot?
A: Southern blotting is a molecular biology technique that is used to detect a person's DNA. This…
Q: What is lac operon system?
A: Lac operon is one of the method by which a prokaryotic cell control gene expression. The lac operon…
Q: Describe the three steps of CRISPR-Cas–mediated antiviral defense.
A: CRISPR stands for Clustered regularly interspaced short palindromic repeats and Cas stands for their…
Q: A specialized plasmid vector that has an E.coli promoter upstream of a restriction site into which…
A: Vectors are DNA molecules that act as transporting vehicle which carries foreign DNA into a host…
Q: Figure 5 shows the lac operon structure in Escherichia Coli What will happen if substance S is…
A: Lac operon contains genes coding for proteins in charge of transporting lactose into the cytosol and…
Q: Which of the following are true for the c-myc gene? Select all that apply
A: Myc is a group of regulator genes that code for transcription factors. c-myc is one of the genes in…
Q: Briefly Explain different applications of western blotting. Please explain at your own words.
A: A western blot is an analytical molecular biology laboratory method that is used in the detection of…
Q: What is the relationship between the rpoS gene and the SOS repair mechanism? Briefly explain.
A: In response to the severe DNA damage; many cells have the mechanism which enable them to synthesize…
Q: Where did western blot get its name?
A: The western blot (also known as the protein immunoblot) or western blotting is molecular biology and…
Q: What is Gene therapy – Illustrate using example of Adenosine deaminase deficiency?
A: Genetic engineering is a process through which the desired gene of interest is introduced into the…
Q: Why does the lac operon include more than one operator sequence?
A: Lactose operon (lac operon) is an operon required for the transport and metabolism of lactose in…
Q: What structural features does the trp operon share with the lac operon?
A: Genes are collections of nucleotides that code for a specific protein. For protein synthesis to…
Q: Describe the steps in Southern blotting.
A: Southern blot is a technique used to analyse information about identity, size, and abundance of DNA.…
Q: Give me an examples, when we used reverse transcription.
A: Reverse Transcription is the process of conversion of the RNA into DNA. This process is done by…
Q: Before inserting the blunt-ended PCR amplicon to a dephosphorylated vector, the amplicon is treated…
A: PCR or polymerase chain reaction is a technique used to amplify a given set of DNA fragment using…
Q: How many RNA primers are need for the leading
A: The act of copying a cell's DNA, or DNA replication, is no easy task. Your genome contains…
Q: For each of the growth conditions listed, what proteins would be bound to the lac operon? (Do not…
A: An operon consists of many related genes that are regulated by a common operator. The multiple genes…
Q: "CRISPR-Cas Is an Adaptive Immune System in Bacteria" Explain this ?
A: Bacteria is one of the common known microscopic organisms. An organism that can not be seen through…
Q: The lac operon is an inducible operon. What does this mean?
A: Operons are the groups of genes related to a single pathway and controlled by a single promoter.…
Q: Why is generalized transduction able to transfer anygene, but specialized transduction is restricted…
A: Introduction: transduction is a process of gene transfer in bacteria from donor to recipient…
Q: Does CRISPR CAS9 as a defense in bacteria only work for double stranded viral DNA? or any type of…
A: Scientists can use CRISPR to persistently edit genes in living cells and creatures and, in the…
Q: Give all possible genotypes of a lac operon that produces, or fails to produce, B-galactosidase and…
A: The diagrammatic representation of lac operon is given below: -
Q: a student uses a monoclonal antibody to detect a protein using a direct Western-blotting protocol…
A: Secondary antibodies are used to detect a target after a particular primary antibody has been bound…
Q: On this gene map, there are sections where large chunks of the same color are present. These might…
A: The gene or genes which gets transcribed when the operator is bound are known as operons.
Q: Please explain the structure of Lac operon in bacteria.
A: Gene regulation can be defined as any kind of alteration in the gene to give rise to a different…
Q: How does the tryptophan operan turn back on in gene regulation?
A: Bacteria like Escherichia coli (a benign tenant of our gut) need amino acids to survive since they,…
Q: In Northern and Western blotting, what is the purpose of gel electrophoresis?
A: Blots are strategies for moving DNA (deoxyribonucleic corrosive), RNA (ribonucleic) and proteins…
Q: What is Ponceau S and why do we use it during western blotting?
A: Western Blotting : It is an analytical technique of molecular biology which is used to separate the…
Q: What is the difference between the role of the operator and the role of the promoter in an operon
A: According to Jacob and monod concept, operon is a group of contiguous structural genes showing…
Why is phospholipase c is the target on this western blot explain in detail
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- Very briefly, explain how a ligand binding to the extracellular receptor region of a GPCR causes a change in biological events inside the cell.Which of the following domains would you not expect to see in the receptor for BMP4? A. A ligand-binding domain B. A transmembrane domain C. A cytoplasmic domain D. A DNA-binding domain E. All of the above would be seen in the receptor for BMP4You have isolated a new species of infectious bacteria. The bacterium releases a toxin that you believe is adversely affecting heterotrimeric Gs (stimulatory)-protein-based signaling. To explore this hypothesis you use an epithelial cell line that is expressing a cyan fluorescent protein (CFP)-labeled α subunit and a yellow fluorescent protein (YFP)-labeled β subunit of a heterotrimeric Gs-protein. CFP emits blue light and has excitation and emission wavelengths of 440 nm and 490 nm, respectively. YFP emits yellow light and has excitation and emission wavelengths of 490 nm and 527 nm, respectively. To test your hypothesis, you perform two experiments. First, you apply a signaling ligand known to activate this Gs protein and track yellow fluorescence. Second, you apply the signaling ligand and the purified bacterial toxin simultaneously and track yellow fluorescence. Which of the following conclusion will you draw based on the above experimental data? The toxin locks the α subunit…
- Fluorescence-activated cell sorting (FACS) is a powerful technique for separating cells according to their content of particular molecules. For example, a fluorescence-labeled antibody specific for a cell-surface protein can be used to detect cells containing such a molecule. Suppose that you want to isolate cells that possess a receptor enabling them to detect bacterial degradation products. However, you do not yet have an antibody directed against this receptor. Which fluorescence-labeled molecule would you prepare to identify such cells?Pathway analysis: Link the protein names to the correct statements by interrogating the depicted pathway. Options: A. RTK Receptor Threonine Kinase B. GEF Guanidine Exchange Factor C. PLC Phospholipase C D. GAP GTPase Activating Protein E. Gαi F. Raf Rapidly Accelerated Fibrosarcoma G. PAK1 p21 Activated Kinase H. WASP Wiscott Aldrich syndrome protein I. RTK Receptor Tyrosine Kinase J. GPCR G-protein Coupled Receptor K. Steroid Receptor L. Phosphatase M. MEK1 (Mitogen-Activated Protein) Kinase/ERK (Extracellular Signal-Regulated Kinase) Kinase 1 N. AC Adenylyl cyclase O. IP3R IP3 Receptor P. ERK1/2 Extracellular Signal-Regulated KinaseContinuous exposure of a Gαs protein coupled receptor to its ligand leads to a phenomenon known as desensitization. Describe several molecular mechanisms for receptor desensitization. How can a receptor be reset to its original sensitized state? What effect would a mutant receptor lacking serine or threonine phosphorylation sites have on a cell?
- Cyclic adenosine monophosphate (CAMP) is a molecule that can act as anallosteric activator of kinase enzymes inside of the cytoplasm. Describehow CAMP molecules affect kinase enzymes?Explain, in detail, how tyrosine kinase proteins are involved in one signal transduction pathway of your choice. Make sure you describe the complete pathway in which it is involved. Then, describe how you would experimentally demonstrate the requirement of a tyrosine kinase protein in your chosen pathway.Name and define (briefly) the four different types of receptors involved in Cellsignaling. Give an example of a ligand and specific type of receptor (where would you find the receptor?) for one of the receptors defined.
- Members of the integrin family are the major cell surface receptors for many ECM proteins such as fibronectin. In experimental studies, single integrin heterodimers have been found to assume more than one structural conformation. Describe the relationship between integrin structures and their activities.Between direct and indirect allosteric kinase inhibitors, which do you think requires a larger conformational change in response to binding of the allosteric modulator? Why?Two proteins have exactly the same amino acid sequence. Protein A is able to bind with high affinity to a cellular receptor, while Protein B cannot bind to the receptor under identical conditions. In 3-4 sentences explain, provide an explanation why two proteins with the same primary sequence could have very different abilities to bind a receptor.