Why is the euchromatic region or light bands in G banding rich in Guanine and cytosine? And why is the heterochromatic region or dark bands rich with adenine and thymine?
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- In pyrimidine nucleosides, the anti conformation predominates. Explain. Do the purine nucleosides have similar interactions?Why is R-banding significant when its banding pattern is just the reverse pattern of G-banding?What does the high-energy molecules adenosine triphosphate fforms and reduced forms of nicotinamide adenine dinucleotide means?
- Why is the pKa of the arginine side chain greater than the pKa of the lysine side chain?In Figure 1-8b, can you tell if the number of hydrogenbonds between adenine and thymine is the same as thatbetween cytosine and guanine? Do you think that aDNA molecule with a high content of A + T would bemore stable than one with high content of G + C?Given the fragment Ala-Lys-Tyr-Gly-Phe and Leu-Val-Ser-His-Cys-Arg, what is the expected result in both fragments with CuSO4 and NaOH? a. dark violet solution b. light violet solution c. blue solution d. colorless solution
- A gene contains 30% thymine. What is the percentage of pyrimidines present in this segment? Explain.For the trinucleotide 5’ G-C-A-3’ How many nucleotide subunits are present in its ‘backbone’? How many nucleotide ‘non-backbone’ subunits are present? How many phosphodiester linkages are present? What is the overall charge carried by the trinucleotide?In a-c below, will the residue on the right-hand side increase or decrease the pKa of the residue on the left-hand side? Explain.