Why must materials and instruments used in tissue culture be kept sterile?

Q: Are any cellular structures other than amyloplasts stained intensely by iodine?

A: An amyloplast is a plastid type of organelle which produces and stores starch-like materials in the…

Q: nd cell line same

A: Cell culture is that the culture of cells is directly isolated from parental tissue of interest;…

Q: Approximately how many bacterial colonies are present on plate B?

A: Bacteria are small, microscopic, and unicellular organisms that are found almost everywhere on the…

Q: Why does treatment of human skin with alcohol not create a completely sterile environment?

A: Sterilization is the process of getting rid of all the germs on the surface of the object and makes…

Q: How does subcellular centrifugation exactly work? At slower speeds, do the smaller/less dense…

A: Introduction: Subcellular centrifugation is a centrifugation technique that is used in the isolation…

Q: What are micro-propagation in tissue culture?

A: Tissue culture is the process of growing cells or tissues in an artificial medium independent from…

Q: How could colony-stimulating factors be used as a therapy?

A: colony-stimulating factors are used as a  many therapies :

Q: What are the main characteristics of scanning electron microscopy?

A: Electrons are the negatively charged particles having minus 1 charge  and represented by e- ,…

Q: What is the charge of the target cellular structure of positive staining and negative staining?

A: Basic stains are cationic when it is ionized, the chromogen exhibits a positive charge. the positive…

Q: What do you mean by ultrafiltration?

A: The excretory system is the system that performs excretion, the bodily process of discharging…

Q: What is secondary cell culture?

A: Secondary culture is a subculture of the primary culture. All the cells grown in the Petri dish…

Q: What is the usage of the superresolution microscopy technique?

A: The microscope is identified as an optical instrument to observe very small objects. It is able to…

Q: Why must primary cell cultures be restarted every so often when preparing primary cell cultures to…

A: Primary cell cultures are derived from tissue and they are more similar to the in vivo state and…

Q: Write the procedure for celloidin embedding.

A: Hi! Thanks for your question. But as you have posted multiple questions, I am answering the first…

Q: What are types of cell culture media?

A: A cell culture medium is solid or liquid in nature. It is a mixture of all necessary nutrients…

Q: What are plaque-forming units?

A: A plaque-forming unit (PFU) is a measure used in virology to describe the number of virus particles…

Q: What would happen to your check cells if they were extracted in anything other than isotonic…

A: As the name suggests the isotonic solution have a solute concentration same as that of the cell.…

Q: How to prevent MDCK cells from forming cysts in culture?

A: MDCK is also known as Madin-Darby Canine Kidney. The cells are a type of model mammalian cell used…

Q: What is the difference between conventional optical microscopes and inverted microscopes?

A: Introduction Conventional optical microscopes:- This type of microscope uses visible light (400-700…

Q: why is microtome important in microscopy?

A: Objects or organisms that cannot be viewed by the unaided eyes are studied under the microscope.…

Q: What is immunofluorescence microscopy

A: Immunofluorescence microscopy is a technique used for light microscope with a fluorescent microscope…

Q: Why is plate count more sensitive than a microscopic count?

A: Microorganisms such as bacteria are cultured artificially in laboratories, in order to study their…

Q: What is a microscope? What is its significance in the Medical Technology profession?

A: Microbiology is the study of microorganisms, which are unicellular or cell-cluster microscopic…

Q: What is a step-by-step process of preparing and inspecting a microorganism under a microscope?

A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…

Q: What is the difference between a scanning and a transmission electron microscope? When do you use…

A: Microscopes are the instruments that allow scientists to study objects that cannot be seen through…

Q: Why are chemical stains required for visualizing cells and tissues with the basic light microscope?

A: Cell staining is a technique that is used to better visualize cells and cell components under a…

Q: in flow cytometry, when cells labeled with fluorescent molecules pass through the focused laser…

A:

Q: What should be considered when preparing a monolayer cell culture?

A: Monolayer culture, also known as adherent culture, is a form of animal cell culture in which cells…

Q: laboratory? explain with diagram

A: The tissue culture is the general term for the removal of the cells, tissues or organs from an…

Q: why do we used Living cell microsope for?

A: Microscope is an instrument that is used to observe objects that are too small to be seen by our…

Q: What is the advantage of cryo-sectioning for antibody staining methods?

A: To study the antigenic structures, present on the cell surface tissues can be embedded in paraffin…

Q: What causes enuresis ?

A: ENURESIS- Enuresis is the inability to control urination on a regular basis. The word is often…

Q: does the rate of diffusion correspond with the molecular weight of the dye?

A: The rate of diffusion correspond with the molecular weight of the dye...

Q: Which Two types of electron microscopy are in routine use in microbiology? Explain

A: The electron microscope is an instrument that uses the beam of electrons to examine objects on a…

Q: what is the source of illumination for a light microscope?

A: A microscope is an optical instrument used to view objects, for example, animal or plant cell, that…

Q: In monolayer cell culture conditions contact inhibition occurs when the following conditions are…

A: Contact inhibition is a process of arresting cell growth. When cells come in contact with each other…

Q: Why is the resolution so superior in the electron microscope?

A: A beam of electrons that travel in wavelike patterns when accelerated to high speeds form the images…

Q: cell lysate? What is the purpose of the cheesecloth

A: Lysis alludes to the separating of the cell, frequently by viral, enzymic, or osmotic systems that…

Q: How can bacterial growth be retarded?

A: The bacteria are microscopic organism which are seen only under microscopes. They are present…

Q: Why is cell imaging a preferred methos for studying cells

A: Cell imaging a preferred method for studying cells because it enables the visualization and…

Q: Why should freshly collected samples be used for microscopic examination?

A: The benefit of the microscope is that it produces a better resolution than the naked eye. The higher…

Q: Name the structure where ultrafiltration takes place?

A: Step 1 Nephric filtrate consists of water, various electrolyte, glucose, amino acids. It is alkaline…

Q: Would a syringe filter with a pore size of 0.22 micrometers be able to completely remove all…

A: Filtration refers to the sterilization technique that eliminates the microorganism. The fluid that…

Q: what are two advantages and disadvantages of electron microscopes?

A: Introduction: An electron microscope is a microscope that uses accelerated electrons as a source of…

Q: What are the differences between “primary cell culture” and “continuous cell lines”?

A: Primary cell culture is the culture of cells directly isolated from parental tissue of interest.…

Q: What is the function of staining in light microscopy?

A: The light microscopy or light microscope represents a laboratory instrument required for the…

Q: The primary aim of fixation is to preserve the morphological and chemical integrity of the cell.…

A: Fixation is a process by which biological tissue are processed with specific type of fixatives, so…

Q: Write the procedure for celloidin  embedding

A: Answer: Celloidin = It is basically the semisolid solution of pyroxylin in ether or alcohol, which…

Q: Staining is often needed to distinguish the structure of cells as most cells are almost transparent.…

A: Cells are the basic structural and functional unit of life.

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.