How does subcellular centrifugation exactly work? At slower speeds, do the smaller/less dense objects form a pellet first? How do differing speeds lead to different pellet formations?

Q: What are density-gradient centrifugation

A: The process of centrifugation is widely used in laboratories to separate different substances on…

Q: Beginning with 10 grams of plant tissue, order the following procedures (as steps 1-4) that you will…

A: Plant tissue is a cluster of related cells that work together to fulfil a specific purpose for the…

Q: How could you isolate mitochondria from liver cells using differential centrifugation?

A: Isolated mitochondria are used to perform several functions. This organelle is used to study…

Q: What salt did Meselson and Stahl use for their ultracentrifuge gradients? O NaCI O NH4CI O CSCI O…

A: Given: DNA replicates semiconservatively. Matthew messelson and Franklin stahl performed an…

Q: What if the purpose of bacterial culture? Why might it be useful to grow bacteria?

A: According to question what is  the purpose of bacterial culture. Why might it be useful to grow…

Q: What is the similarities of differential centrifugation and gradient centrifugation?

A: Similarities between differential centrifugation and gradient centrifugation is process involved…

Q: What is the size of E. coli bacteria?   What cellular organelle(s) are of a similar size to E.coli?…

A: Escherichia coli  This is a Gram negative bacteria. It is a facultative anaerobic with rod shape…

Q: What is secondary cell culture?

A: Secondary culture is a subculture of the primary culture. All the cells grown in the Petri dish…

Q: What are bacterial colonies and how are theyformed?

A: Solid media or medium is a liquid or gel which is designed in such a way to provide support for the…

Q: how do you graph change over time for onion, cheek , and bacterial cell? Can you explain how you…

A: Onion cells, human cheek cells, and bacterial cells are subjected to be affected by any change in…

Q: Bacterial techniques, plasmid isolation, digestion and gel electrophoresis Answer all with…

A: 1) Lysogeny broth (LB) is a nutritionally rich medium primarily used for the growth of bacteria.…

Q: What is the difference between the arithmetic number (exact or accurate number) and logarithmic…

A: Growth is irreversible change in size, mass or volume of the living organism or different parts of…

Q: Why do microbiologists use cultured plates with 30 to 300 colonies used for calculations?  2. Why…

A: 1. Microbiologists generally used cultured plates with 30 to 300 Colonies for calculation purposes.…

Q: Dilute cells from an old culture 1:200 into 5 mL of fresh media. What volume of the old culture…

A: Introduction Dilution is the process of lowering the concentration of a solute in a solution by…

Q: What are types of cell culture media?

A: A cell culture medium is solid or liquid in nature. It is a mixture of all necessary nutrients…

Q: How can we steralize Animal cell in culture?

A: Sterlisation is the process of removal of any microorganisms present in vegetative or spore form.…

Q: Plaque assay (Bacteriophage Titer) Answer questions 2 & 3 2. Why don't bacteriophages continue…

A: Plaque assay is one of the widely used approaches for determining the quantity of infectious virus…

Q: Describe in full the technique of density gradient centrifugation?

A: Introduction: Centrifugation is a cycle used to separate or pack materials suspended in a fluid…

Q: PART B Re-watch the video and explain why the following steps are necessary: • Why use tweezers to…

A: Introduction  Antibiotics are antimicrobial substances that are effective against microorganisms.…

Q: What is a Conditioned cell-free medium(CCFM)?

A: Culture medium is a liquid or gel designed to support the growth of microorganisms.  There are…

Q: How does gel filtration work? What are the applications of analytical versus preparative versions…

A: Chromatography is a technique for separating a mixture in the laboratory. The mixture is dissolved…

Q: What other method could be used to purify lysozyme from white using its unusual pl?

A: The unique charge properties of lysozyme with a very high pI of about 10.5 can be used in the…

Q: if 250 colonies are present on a pour plate made from10^5 dilution, how many colonies should grow on…

A: Serial dilution method is the process of diluting our culture in a sequential manner. This is done…

Q: what is means cross-contamination in animal cell culture?

A: Biological cells can be grown and developed in laboratory conditions by cell culture techniques. It…

Q: subcellular fractionatio

A: Centrifugation:  Molecules with different densities are separated by rotating them in a solution…

Q: Why does a one-step growth curve differ in shapefrom that of a bacterial growth curve?

A: Growth curves are generally the description of the density of cell populations in liquid culture…

Q: temperature and light source affect the number and type of colonies that can be found in the…

A: Microbes such as bacteria, fungi, viruses are grown at the earth's surface. The atmosphere provides…

Q: Write in details about types of gel ?? 2. Levigating agent role in Suppositories manufacturing?

A: A levigating agent is defined as a liquid that is viscous and has a low surface tension in order to…

Q: PART B Re-watch the video and explain why the following steps are necessary: Why use tweezers to put…

A: Please note as per the site regulations, external links are not watched. Hence, part A cannot be…

Q: What are the kinds of microtome with their corresponding type of embedding medium?

A: A microtome is a cutting tool that creates incredibly tiny material slices called sections.…

Q: How is true microbial motility distinguished from other kinds of motion like Brownian movement that…

A: Q. How is true microbial motility distinguished from other kinds of motion like Brownian movement…

Q: Bacterium Q had the lab results below. What can you tell me about the protein metabolism of this…

A: Test results Gelatin tube- Solid Urea agar slants- fushia Phenylalanine slants- no color Peptone…

Q: Beginning with 10 grams of plant tissue, order the following procedures (as steps 1-4) that you will…

A: Cell organelles extraction  The process to obtain cell organelles from the cell.

Q: What are the advantages of using microorganisms in producing Single Cell Protein?

A: -Single cell proteins or microbial proteins can be described as the proteins referring to edible…

Q: Using a schematic diagram, summarize the following steps in preparing competent cells for…

A: prepare competent cells for transformation ;- Add 20 µL of each ligation reaction or 1 ng of uncut…

Q: What are bacteria? what is bacterial culture? how to grow bacteria and why? why are there so many…

A: As per the honour code, we are entitled to do only three subparts of a multipart question. So, i am…

Q: How would the slide appear if the bacteria were stained with safranin, a basic red dye?

A: The appearance of bacteria, when stained with safranin, is to be determined.

Q: why the Gram stain can differentiate between Gram positive and Gramnegative cells?

A: *Gram staining is a technique used to differentiate two groups of bacteria into gram positive and…

Q: Why differential centrifugation can cause contamination in pellet?

A: Differential centrifugation is a typical process used in microbiology and cytology to separate…

Q: In which solution did potato cell intracellular fuild reach equilibruim with the solution?

A: * During potato experiment we can see the potato may shrink and may swell and may be in no change it…

Q: Would a red blood cell placed in a 200mM sucrose solution lyse?

A: Osmosis is a process in which water molecules moves from the low concentration of solute to the…

Q: What distinguishes Acid-Fast bacteria in terms of cell wall / coating and other characteristics?…

A: Acid-fast bacteria It is the acid-fast bacilli. These are the bacterial group that has the…

Q: why do we used Living cell microsope for?

A: Microscope is an instrument that is used to observe objects that are too small to be seen by our…

Q: What is a complex medium? and What are the basic components of a culture medium?

A: Medium is defined as the solid or liquid substratum on which the cells or organ can grow for further…

Q: Why is a microtome an indispensable tool in a Microtechnique laboratory?

A: By definition, a microtome is an instrument that allows the production of extremely thin slices of…

Q: Which organelle is isolated at the slowest speed on a centrifuge? Which organelle is isolated last…

A: Isolation of cell organelles can be done by centrifugation method as in the subcellular…

Q: How many colonies are present in this plate?

A: Microbiology is the study of microorganisms and associated topics. Microbiology has gone a long way…

Q: Would a syringe filter with a pore size of 0.22 micrometers be able to completely remove all…

A: Filtration refers to the sterilization technique that eliminates the microorganism. The fluid that…

Q: What are the differences between “primary cell culture” and “continuous cell lines”?

A: Primary cell culture is the culture of cells directly isolated from parental tissue of interest.…

Modes of Nutrition

It is a biological process of providing or obtaining food that is necessary for growth and health. It includes many physiological processes - absorption, ingestion, assimilation, catabolism, excretion, etc. In biology, the science that deals with nutrition's physiological and chemical processes are called "nutritional science."