What is the charge of the target cellular structure of positive staining and negative staining?

Q: Are any cellular structures other than amyloplasts stained intensely by iodine?

A: An amyloplast is a plastid type of organelle which produces and stores starch-like materials in the…

Q: When is fluorescence used in a clinical laboratory?

A: Fluorescence is a type of photoluminescence where light is absorbed by an excited molecule or atom…

Q: How does subcellular centrifugation exactly work? At slower speeds, do the smaller/less dense…

A: Introduction: Subcellular centrifugation is a centrifugation technique that is used in the isolation…

Q: What is Circular Dichroism? Discuss its theory and applications.

A: Circular dichroism is a form of light absorption spectroscopy. This method was first described by…

Q: What are micro-propagation in tissue culture?

A: Tissue culture is the process of growing cells or tissues in an artificial medium independent from…

Q: For a stain to be considered differential, what must it do?

A: Differential stain uses more than two stains.

Q: Why is the wavelength of the light that is released in spectrophotometric analysis longer than the…

A: Some molecules absorb light at a particular wavelength and emit light in higher wavelength and this…

Q: How is xylol used to clean the lens of a microscope? What may happenif xylol is constantly used for…

A: The microscope can be defined as the laboratory instrument which is utilized to examine objects that…

Q: Basic stains are more effective for bacterial staining than acidic dyes. Why? Which component on the…

A: Answer 1)Basic stains , which carries the postive charge chromogen are preferred because the…

Q: Are the structural differences between the cell walls of Gram-positive and Gram-negative bacteria…

A: The gram-positive and negative cells differ in their cell wall composition.

Q: What are bacterial colonies and how are theyformed?

A: Solid media or medium is a liquid or gel which is designed in such a way to provide support for the…

Q: Why are bacterial cells generally stained for microscopic viewing?

A: Staining bacterial cells prior to microscopic viewing is a routine process in most of microbiology…

Q: What stain would you use to differentiate between bean and ant cells? Explain and include your…

A: Staining is a procedure that involves the application of colored chemicals called dyes to specimens.…

Q: How do gram-positive and gram-negative bacteria differ in cellular structure, and how does this…

A: Gram staining is a most important  technique in the microbiology to differentiate the group of…

Q: Why must primary cell cultures be restarted every so often when preparing primary cell cultures to…

A: Primary cell cultures are derived from tissue and they are more similar to the in vivo state and…

Q: How can we steralize Animal cell in culture?

A: Sterlisation is the process of removal of any microorganisms present in vegetative or spore form.…

Q: What are the Four types of flagellar arrangements?

A: A flagellum may be a lash-like appendage that protrudes from the cell body of sure cells termed as…

Q: what is means cross-contamination in animal cell culture?

A: Biological cells can be grown and developed in laboratory conditions by cell culture techniques. It…

Q: What stains best enhance the visibility of cellular structures?

A: Microorganisms are minute organisms with colorless cellular structures. They are visualized under…

Q: Which components of the nucleus stains with basophilic dyes?

A: Basophilic means "base loving". The dyes that stain components that are base-loving are called…

Q: What step normally associated with staining bacterial cells is omitted when the dimensions of cells…

A: Step 1 Gram staining is a technique of staining that is used to differentiate and categorize…

Q: What is immunofluorescence microscopy

A: Immunofluorescence microscopy is a technique used for light microscope with a fluorescent microscope…

Q: Why must materials and instruments used in tissue culture be kept sterile?

A: Sterilization of explants is a key step in plant tissue culture.

Q: what kind of microscope is used for cellular structure and why?

A: Microscopes are scientific instruments used to investigate items that are too small for the naked…

Q: What is a step-by-step process of preparing and inspecting a microorganism under a microscope?

A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…

Q: What's the theory and conclusion of a acid fast stain?

A: It was Ziehl who originally devised differential staining procedures, that were later refined by…

Q: Is K2EDTA same as Spray-coated K2EDTA?

A: K2EDTA stands for dipotassium ethylenediaminetetraacetic acid and is a strong anticoagulant used…

Q: How is a cell membrane identified when using a brightfield microscope?

A: Bright field microscope is a simple light microscope which is routinely used in laboratories.

Q: What structure in eukaryotic cells is more easily seen in DICthan in bright-field microscopy?

A: DIC refers to the differential Contrast Microscopy is one of the types of light microscopy and…

Q: in flow cytometry, when cells labeled with fluorescent molecules pass through the focused laser…

A:

Q: How does viability staining differ from stains like DAPI?

A: Staining is the technique in biology, which increases the visibility at the microscopic level. Dyes…

Q: How would the slide appear if the bacteria were stained with safranin, a basic red dye?

A: The appearance of bacteria, when stained with safranin, is to be determined.

Q: why the Gram stain can differentiate between Gram positive and Gramnegative cells?

A: *Gram staining is a technique used to differentiate two groups of bacteria into gram positive and…

Q: What is the secondary stain in the spore stain?

A: Endospores are the structures that are formed by bacteria during unfavorable conditions (lack of…

Q: What is the advantage of cryo-sectioning for antibody staining methods?

A: To study the antigenic structures, present on the cell surface tissues can be embedded in paraffin…

Q: what staining method is used to visualize or detect golgi apparatus?

A: Staining is a technique for making transparent cells or thin pieces of biological tissue more…

Q: What types of compounds make up the gels used in electrophoresis?

A: The types of macromolecules usually separated on Agarose gel are : Nucleic acids-mainly DNA…

Q: does the rate of diffusion correspond with the molecular weight of the dye?

A: The rate of diffusion correspond with the molecular weight of the dye...

Q: define the term multicolor banding,

A: Chromosomes are long thread-like structures that carry coded genetic information in the form of DNA.…

Q: What macromolecules does DAPI stain? What are its advantages and disadvantages?

A: Genetics is the branch of biology that deals with genetic material like DNA, RNA, inheritance.…

Q: Aside from H&E, what other staining methods are applicable in Golgi apparatus, mitochondria, and…

A: Answer :- Subsequent to trying different things with presenting cells to gold and mercury, Golgi…

Q: Aside from safranin, what other stains can we use for the microscopic examination of onion cells?

A: We can not see all the part of a cell by naked eye. With the help of different types of microscope…

Q: What is the formula used to describe the resolution of a microscope lens, and what are the…

A: The term resolution is used in microscopy to characterize a microscope's capacity to identify…

Q: Why is cell imaging a preferred methos for studying cells

A: Cell imaging a preferred method for studying cells because it enables the visualization and…

Q: What type of chromophore is associated with a negative stain?

A: Bacteria are unicellular prokaryotic organisms that maintain a definite shape since they possess the…

Q: PLS ANSWER ALL 1. Why were nuclei being unstained when you used eosin? 2. Aside from H&E, what…

A: Staining is a technique which is used to find different parts in the cell while observing under a…

Q: What is the principle of image formation on transmission election microscope and scanning electron…

A: The general biological microscope helps in the visualisation of the image of a particular sample…

Q: How is it determined which molecules will enter the cell or remain at the surface?

A: Introduction  All living organisms are made up of cells, which are the basic building components.…

Q: What are the differences between “primary cell culture” and “continuous cell lines”?

A: Primary cell culture is the culture of cells directly isolated from parental tissue of interest.…