Why was it necessary to use phosphoaminophosphonic acid-adenylate ester (ANP) in the ATP synthase enzyme active site rather than ATP to resolve the molecular structure of the ATP synthase active site by x-ray crystallography? C ANP - Mg²+ E193 T164 E189 31 K ANP has a S-S bond in the purine ring of the nucleotide to aid in crystallography. ANP is nonhydrolyzable and will trap the enzyme in the desired conformation. ATP cannot be used in a cell free system because it will be readily hydrolyzed. ATP is twice as large as ANP and will not fit in the ATP synthase active site. ANP is hydrophobic and ATP is hydrophilic, which makes ANP a better reactant.
Why was it necessary to use phosphoaminophosphonic acid-adenylate ester (ANP) in the ATP synthase enzyme active site rather than ATP to resolve the molecular structure of the ATP synthase active site by x-ray crystallography? C ANP - Mg²+ E193 T164 E189 31 K ANP has a S-S bond in the purine ring of the nucleotide to aid in crystallography. ANP is nonhydrolyzable and will trap the enzyme in the desired conformation. ATP cannot be used in a cell free system because it will be readily hydrolyzed. ATP is twice as large as ANP and will not fit in the ATP synthase active site. ANP is hydrophobic and ATP is hydrophilic, which makes ANP a better reactant.
Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter26: Synthesis And Degradation Of Nucleotides
Section: Chapter Questions
Problem 14P: The Energy Cost of dTTP Synthesis (Integrates with Chapter 20.) Starting from HCO3, glutamine,...
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