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- Please write introduction paragraph on the topic about about human germline gene editing using CRISPR-Cas9.write the pros and cons of crispr technology in bullet points with explaintionConsider two transgenic organisms (microorganism, plant, animal). Diagram the procedure on how the two transgenic organisms were created. Indicate the gene introduced and the procedure to create the said transgenic organism.
- Please help explain Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screenPlease describe the detailed procedures of southern blot. Explain the protocol and principle of restriction fragment length polymorphism (RFLP) and how RFLP is used for genetic disease diagnosis.Based on the given transgenic organism, give a brief explanation on the alterations of the organism
- How do you use CRISPR to treat a patient with sickle cell anemia? Please provide a detailed explanationWhy is genome editing by CRISPR-Cas advantageous over traditionalmethods for creating knockout or transgenic animals?Explain your answers.Briefly and in simple terms, describe how CRISPR could be utilized in treating genetic conditions such as Batten disease.
- Design a oligonucleotide probe for provided gene sequence using all the guidelines for efficient probe designing. ACAACCCCAAGCCTTCAACCACCCCCTTCCCCCAAATTAGAGATCGATCTCAAGAAGAAGAATGGGTTCCGTCTCTCGCTCTTCTTTGGATCAGAAGCTGGCCATGGCAAAGCGCTGCTCCCACGAGGGAGTTGTCGCGGGAGCAAAGGCGGCCGTGGTTGCAACTGTTGCCTCGGCCATTCCTACTTTGGCTAGCGTTAGGATGATCCCATGGGCGAGGTCCTTCCTTAATCCCGCAGCTCAGGCCCTCATCGTTTCATCAGCGGCGGGGGCGGCGTACTTCATAGTTGCGGACAAGACWrite and discuss about the analysis procedure used for carrier screening for the single-gene diseases that is routinely conducted in different countries. (Subject: Genetic engineering).Using the “target DNA” sequence provided in the attached image, what sizes of inserts can be prepared using HindIII digestion? Which one contains the coding sequence?