You are asked to prepare HEPES buffered saline with the following constituents: НЕРES 10mM KCI 5mM NaCI 135mM CaCl2 1mM M9SO4 0.6mM Glucose 6mM You have previously made up 100 mM stock solutions of HEPES, KCI, CaCl2, and M9SO4 and a 1M stock of NaCl. The MW of glucose is 180.16 g/mol What volume of HEPES would you require to make up 400ml of buffer?

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter9: Parenteral Medication Labels And Dosage Calculation
Section: Chapter Questions
Problem 6.4P
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You are asked to prepare HEPES
buffered saline with the following
constituents:
НЕРES
10mM
KCI
5mM
NaCl
135mM
CaCl2
1mM
M9SO4
0.6mM
Glucose
6mM
You have previously made up 100 mM
stock solutions of HEPES, KCI, CaCl2,
and M9SO4 and a 1M stock of NaCl.
The MW of glucose is 180.16 g/mol
What volume of HEPES would you
require to make up 400ml of buffer?
Transcribed Image Text:You are asked to prepare HEPES buffered saline with the following constituents: НЕРES 10mM KCI 5mM NaCl 135mM CaCl2 1mM M9SO4 0.6mM Glucose 6mM You have previously made up 100 mM stock solutions of HEPES, KCI, CaCl2, and M9SO4 and a 1M stock of NaCl. The MW of glucose is 180.16 g/mol What volume of HEPES would you require to make up 400ml of buffer?
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