You are given two proteins with a pI of 4.5 and pI of 7.7. In an example of an anion-exchange column, can you use a buffer to separate these two proteins? What buffer protein can be used to separate the two proteins mentioned above?
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You are given two proteins with a pI of 4.5 and pI of 7.7.
In an example of an anion-exchange column, can you use a buffer to separate these two proteins? What buffer protein can be used to separate the two proteins mentioned above?
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- The pH of black coffee is 5, and that of milk of magnesia is 10. Is the coffee twice as acidic as milk of magnesia?You have given two proteins with a pI of 4.5 and pI of 7.7? Using an anion-exchange column, how can these two proteins be separated. What buffer could you prepare?If you added three proteins (Protein A, pI 5.0; Protein B, pI 6.0; Protein C, pI 7.0) to an anion exchange column equilibrated with bicine buffer, pH 8.5, in what order would these protein be expected to elute as the column was loaded, washed, and then increasing amounts of NaCl were added?
- Three buffers are made by combining a 1M solution of acetic acid and a 1M solution of sodium acetate in the ratios shown in the table below. Which of these statements is true regarding the prepared buffers? (Ka= 1.7x10-5) pH of buffer 1 < pH of buffer 2 < pH of buffer 3 pH of buffer 1 = pH of buffer 2 = pH of buffer 3 pH of buffer 1 > pH of buffer 2 > pH of buffer 3 pH of buffer 1 = pH of buffer 2 > pH of buffer 3 pH of buffer 1 > pH of buffer 2 = pH of buffer 3Given a stock protein solution with a concentration of 6 mg/ml, determine the protein concentration of a solution made by mixing 5 μl of the stock with 5 μl of a buffer.What is the pH of a buffer of a buffer system that contains a weak acid of .19 M and conjugate base of .12 M ? The Ka of the acid is 5.6x 10 -6 pH= pKa + log ( [A- ]/[HA]) please show me step by step
- Consider the following pH titration curve of a diprotic acid. What is the approximate values for pka 1 and pka 2? the curve is attached below.In an experiment, what happens to the calculated molar mass after waiting an hour between thawing the frozen cyclohexane and adding the unknown compound, during which time some of the cyclohexane evaporated. Will the molar mass be too large, too small, or unaffected? Explain.|You have a 0.5M stock solution of NaCl (formula weight: 58.4 g/mole), a 0.15M stock solution of glucose (formula weight: 180.2g/mole), and a bottle of solid Tris base (formula weight: 121.1g/mole). How would you prepare (be specific) 25 mL of a single solution containing 10mM Tris, 15mM glucose, and 5mM NaCl.