You are making growth media for your favorite bacterium. You have 25X LB (the nutrient mixture) and 15 mg/ml kanamycin. You want to make 400 mL of LB with 30 µg/mL of kanamycin and 1% agar. What quantities of each ingredient do you need to make your media? Be sure to include UNITS! (You will be asked to show your work in the next question.)

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You are making growth media for your favorite bacterium. You have
25X LB (the nutrient mixture) and 15 mg/ml kanamycin. You want to
make 400 mL of LB with 30 ug/mL of kanamycin and 1% agar. What
quantities of each ingredient do you need to make your media? Be sure
to include UNITS! (You will be asked to show your work in the next
question.)
25X LB|
15 mg/ml kanamycin
agar
dH20
Transcribed Image Text:You are making growth media for your favorite bacterium. You have 25X LB (the nutrient mixture) and 15 mg/ml kanamycin. You want to make 400 mL of LB with 30 ug/mL of kanamycin and 1% agar. What quantities of each ingredient do you need to make your media? Be sure to include UNITS! (You will be asked to show your work in the next question.) 25X LB| 15 mg/ml kanamycin agar dH20
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