* You can keep extra decimal places through most of this problem, but round your final estimate to one decimal place* You wish to quantify your favourite compound in an experimental solution using spectrophotometry. First, you use standard solutions of your favourite compound to collect the following standard curve data. Calculate the slope of the line of best fit. Standard curve data
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- Suppose a researcher is investigating the measurement ability of a new device intended to read the freezing point of a chemical compound. The substance used in the investigation has a known freezing point of -24 degrees Celsius. The researcher conducted a series of 10 sample measurements of the freezing point, and the results are represented below. What can we say about the device with regards to its precision, and potential bias? Measurement Freezing Point (degrees Celsius) 1 -26 2 -18 3 -32 4 -31 5 -24 6 -24 7 -24 8 -24 9 -11 10 -37Why is it important that the standard curve you create in biological analyses with spectrophotometry is measured using specialty cuvettes?In regards to an experiment where paper chromatography is used to calculate the Rf number, is this considered a qualitative or quantitaive assessment of the data? Why is it a qualitative or quantitative assessment of the data?
- Pretend you are performing an experiment with a pigmented substance similar to BPB. As the concentration increases, the color of the liquid in the container intensifies. a. How can you use a standard curve to compare the color of your samples? b. What is going the standard curve look like? What are you going to have on your X and Y axes? c. Is your standard curve always be the same if you use the same pigment and the same concentration?You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0 0.225 1 0.310 2 0.420 3 0.510 4 0.610 5 0.720 6 0.810 7 0.915 8 0.950 9 0.980 10 0.990 Set up a standard curve. What would the quality of the line-fit (R2 value) be if you do not exclude experimental outliers? (Give you answer to 4 decimal places) Answer Skip ExitPLease help me with then following and explain. make sure your asner is correct pls and thank you. If you increase the sample size (n) of a sample, will the following values increase, decrease, remain similar, or remain exactly the same? A) Y ̅ B) σ C) s D) SE_Y -
- Can you explain it? How can you determine the answer just by the data provided? Not by plotting any graph. If possible, can you provide the graph?The experiment file says "Fill a cuvette with water and blank the spectrophotometer." What are we doing when we do this? Why is this step importantWhat are the x and y values that you have input in the excel? I thought the x would be from the concentation and the y is from the A595 of sample minus A595 of blank but the linear equation that shows up in mine is different. It's y = 0.0443x + 0.0148.
- Scenario: First, 0.1 mL of the unknown is mixed with 0.1 mL of distilled water. Then, 0.1 mL of the solution and 0.9 mL of distilled water are mixed in a cuvette and the absorbance is measured at 410 nm. Given the absorbance, molecular mass and the extinction coefficient of the unknown are 0.650, 16950 g and 188 mM-1cm-1 respectively, and the path length is 1 cm, how can I find the original concentration of the unknown in mg/mL by Beer-Lambert Law (NOT molarity M)?you know that 1.1OU=1um. you have an organism that measures at 12 O.U at 10x. How long is your organism? assume O.U were calibrated at 1000x.Is it possible to determine Km and Vmax without using excel? Because we are not allowed to use computer during exam, so I dont understand how its done without using excel?