You have a 11 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 8.22. You incubate his sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid dissociates to yield acetate and hydrogen ions. At the end of the incubation period, you measure the pH again and find that it has decreased to 6.62. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in he original 11 mL sample. CH,—C−O−CH,—CH,—*N–CH, Acetylcholine CH 3 acetycholine in original sample: CH3 H₂O CH 3 HO–CH,—CH,—*N–CH, + CH,—C−O + H* CH, Co Choline CH3 Acetate nmol
You have a 11 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 8.22. You incubate his sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid dissociates to yield acetate and hydrogen ions. At the end of the incubation period, you measure the pH again and find that it has decreased to 6.62. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in he original 11 mL sample. CH,—C−O−CH,—CH,—*N–CH, Acetylcholine CH 3 acetycholine in original sample: CH3 H₂O CH 3 HO–CH,—CH,—*N–CH, + CH,—C−O + H* CH, Co Choline CH3 Acetate nmol
Chemistry: The Molecular Science
5th Edition
ISBN:9781285199047
Author:John W. Moore, Conrad L. Stanitski
Publisher:John W. Moore, Conrad L. Stanitski
Chapter15: Additional Aqueous Equilibria
Section: Chapter Questions
Problem 17QRT: Without doing calculations, determine the pH of a buffer made front equimolar amounts of these...
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![You have a 11 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 8.22. You incubate
this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid
dissociates to yield acetate and hydrogen ions. At the end of the incubation period, you measure the pH again and find that it has
decreased to 6.62. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in
the original 11 mL sample.
CH,—C−O−CH,—CH,—*N-CH,
Acetylcholine
CH3
acetycholine in original sample:
I
CH₂
H₂O
CH₂
HO–CH,—CH,—*N–CH, + CH,-C−O + H*
CH₂-8-0
Choline
|
CH3
Acetate
nmol](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fdc07c092-5f49-4d93-b501-b08294061775%2F6439b5b4-b419-4e8a-9290-7588a16f022f%2Fe3c75we_processed.png&w=3840&q=75)
Transcribed Image Text:You have a 11 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 8.22. You incubate
this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid
dissociates to yield acetate and hydrogen ions. At the end of the incubation period, you measure the pH again and find that it has
decreased to 6.62. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in
the original 11 mL sample.
CH,—C−O−CH,—CH,—*N-CH,
Acetylcholine
CH3
acetycholine in original sample:
I
CH₂
H₂O
CH₂
HO–CH,—CH,—*N–CH, + CH,-C−O + H*
CH₂-8-0
Choline
|
CH3
Acetate
nmol
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