You have counted a total of 6.4 x 10° cells /ml in preparation for plating cells at a concentration of 1.5 x 10° cells/ml in a T cell proliferation assay. You need 5 ml of cells for your assay. Calculate the volume of counted cells and the volume of cell culture medium you will need to mix together to prepare the cells for plating. O 2.73 mi of cells and 2.27 ml medium O 21 ml of cells and 2.9 ml medium O 1.17 ml of cells and 3.83 ml medium O 145 ml of cells 3.55 ml medium
Q: Which of the following groups of early-diverging animals is comprised of two layers of cells that…
A: Introduction Divergent evolution:- It refers to the process by which interbreeding species diverged…
Q: Which one of the following organelles is considered as the "energy producing" centre of the cell? A.…
A: Mitochondria are membrane-bound cell organelles that provide the majority of the molecular energy…
Q: Which of the following statements regarding nucleotides is false? A) Nucleotides contain sugar…
A: The nucleotide is the sub unit of nucleic acid. DNA and RNA are the examples of nucleic acid. The…
Q: Which are true regarding tick-borne bacterial diseases? Select all that apply. - One example is the…
A: Tick borne diseases, that affect the human and other animals, are caused by pathogens transmitted…
Q: Which of the following amino acids would you expect to find more often near the center of a folded…
A: Proteins are biomolecules made up of long chains of amino acids. It is macronutrient that is…
Q: why do some rats succesfully learn the moris water maze and some dont. These rats over a large…
A: Morris water maze test is a test commonly used test to study spatial learning and memory in animals,…
Q: State the three main functions of the digestive system.
A: Digestion involves two aspects- mechanical digestion and chemical digestion. Mechanical digestion is…
Q: To explain: Why do seedlings that germinate in a fully darkened room grow taller than seedlings of…
A: Every living thing is hardwired to thrive in its own environment. Nonetheless, they require a number…
Q: What is a clitoris
A: It is the most sensitive region in the female reproductive system. It is a bulb shaped sexual…
Q: 160 140 120 100 80 60 40 20 1845 1865 1885 1905 1925 Time (years) (a) Based on the graph, identify…
A: The relationship between the lynx and the snowshoe hare is a classic example of the predator-prey…
Q: Invasive species fishzilla How is it controlled?
A: Introduction Invasive species:- It is an organism that causes ecological or economic harm…
Q: Section E. The Secondary Tissues of Roots in Woody Dicots Identify and label these parts in old…
A: Introduction In vascular plants, a vascular bundle is a component of the transport system. The…
Q: Sam was found to be heterozygous for sickle-cell (Ss) trait. The alleles represented by the letters…
A: Sickle cell is the most common form of abnormal haemoglobins. It occurs due to missense mutation in…
Q: Which of the following statements regarding RNA is false? O A) RNA is a nucleic acid. O B) One RNA…
A: RNA stands for Ribonucleic acid. It is one of the genetic material present in the cell. The whole…
Q: ACTIVITY 12 - LIPIDS As you watch the video, take notes about what you are learning about lipids.…
A: Introduction A lipid is any of several chemical molecules that are water insoluble. They are…
Q: Which among the following statements is NOT an important function of skeletal system? OA. calcium…
A: Skeletal system is the body's main central framework. It consists of bones, connective tissues such…
Q: explain the relationship between biological race, ancestry, and socially defined race
A: Classification of organisms is done using morphological, physiological, and molecular data along…
Q: PROTEINS NUCLEIC ACIDS
A: Proteins serve a variety of purposes, such functioning as enzymes and hormones, regulating fluid and…
Q: Hello I need one more main point for my third medical paragraph about the uses of marijuana in…
A: INTRODUCTION Marijuana (cannabis) is a dried, shredded leaf, stems, seeds, and flowers combination…
Q: What is the name of the mechanism that ensures that there is a higher concen- tration of sodium ions…
A: Introduction - Because the phospholipid bilayer is impermeable to charged atoms, ion channels…
Q: Mate the F1 generation with each other to produce F2 offspring.
A: Two strains- eye color and body color Parents- red eye and green body× yellow eye and brown body…
Q: Several proteins involved in DNA repair are used in multiple pathways. Which one of the following is…
A:
Q: An allosteric activator or inhibitor
A: The allosteric enzymes functions through reversible, noncovalent binding of regulatory compounds…
Q: After tmRNA releases an mRNA and aberrant polypeptide from a stuck ribosome, the resulting…
A: Once mRNAs enter the cytoplasm they are translated stored for later translation or degraded. mRNAs…
Q: 2) Cows were domesticated from wolves approximately 20,000 years ago, likely in Europe . Mutation…
A: Natural selection is an evolutionary process proposed by Charles Darwin in the year 1859. His theory…
Q: 4. Write short notes on Ribosome.
A: Introduction - The site of protein synthesis in the cell is the ribosome, which is an intercellular…
Q: Which of the following characteristics is true for the inner section of a membrane? O hydrophilic O…
A: Introduction Lipids:- Lipids are oily or greasy nonpolar molecules, stored in the adipose tissue of…
Q: Mitochondria produce which of the following? A. ATP B. DNA C. RNA D. proteins
A: Answer :- Option A correct, Mitochondria which is produce ATP (adenosine tri phosphate) ATP are…
Q: 17. Lariat formation is a step in which of the following: transcription post-translation…
A: Lariat formation is a step in transcription.
Q: A 43-year-old man is diagnosed with chronic myeloid leukemia (CML) and started on standard imatinib…
A: Man with Chronic myeloid leukemia treated with imatinib therapy :-
Q: greater omentum [Choose Jh [ Choose] Parietal peritoneum holds the small intestines medial borrder,…
A: Digestive system is responsible for uptaking of food and digestion and absorption of food. The…
Q: Genes are made by- (A) Histones (B) Lipoproteins (C) Hydrocarbons (D) Polynucleotides
A: Introduction - A gene is a basic unit of heredity in biology, as well as a sequence of nucleotides…
Q: Describe the the levels of chromatic packing in an interphase nucleus.
A: Introduction Chromatin is a DNA-protein complex found in eukaryotic cells. The principal role is to…
Q: Give an example of a phantom sensation that can be experienced (it can be any one of your senses).
A: The physical process during which our sensory organs those involved with hearing and taste, for…
Q: Which of the following lists the three steps of translation in their proper sequence? O 1.…
A: Translation is defined as the process of protein synthesis. It takes place in the cytoplasm.
Q: The following step takes place in the Krebs cycle (actually it's two steps but we will assume it is…
A: Krebs cycle is also known as citric acid cycle or tricarboxylic acid cycle. In prokaryotes, it takes…
Q: 8. DNA basic structure: Below, I have drawn a representation of a single strand of DNA. Draw a…
A: The self replicating molecules that are present in the chromosomes and carry genetic information are…
Q: Why is glucose provided by glycogenolysis in the liver but not in skeletal muscle?
A: Through glycogenolysis process, glycogen broken down into glucose and provide energy. Muscle…
Q: The human axial skeleton includes O A. Femur (thigh) O B. ossicle OC TIbia & Fibula (Leg) O D.…
A: The skeletal system is your body's central framework. It consists of bones and connective tissue,…
Q: Which of the following is not part of a carpel? O anther O style O stigma O ovary
A: The central whorl of a flower is made up of leaflike, seed-bearing components. The pistil is made up…
Q: . .is the arrival of pollen on a receptive stigma. ... .
A: A flower is the reproductive part of an angiosperm plant's modified thick shoot. The transfer of…
Q: C. leukocidin 4. Today approximately 90% of S. aureus strains are resistant to A. methicillin. B.…
A: 4. When bacteria are first exposed to an antibiotic, those which are most susceptible to the…
Q: Manzanita comes in a large variety of species and forms. Which statement is true? each chaparral…
A: To determine: To determine the correct option of manzanita species from the given set of options.
Q: sketch the microscopic features of the genus asperigillus
A: Introduction :- A fungus is any member of a group of eukaryotic organisms that includes…
Q: Which of the following is NOT true of miRNAs? A. Pri-miRNAs are processed by Drosha in the…
A: A microRNA, also known as a miRNA, is a single-stranded, non-coding RNA molecule that is very small.…
Q: Robert Koch (a) proposed a set of guidelines to demonstrate that a specific pathogen causes specific…
A: Microorganisms are small living organisms some of which are pathogenic and cause diseases.
Q: Assume you want to make and study fragments of a protein. Would you expect that any fragment of the…
A: Proteins are the polymers of amino acids.
Q: Describe the timeline of an acute infection, particularly naming which cells are the first to arrive…
A: Acute infections are infections that are caused suddenly by any condition or by pathogens such as…
Q: Bacteria use the following mechanism(s) to resist antibiotics:
A: Antibiotic resistance Antibiotic are the class of compounds that inhibits the growth of bacteria or…
Q: In sulfur photosynthetic bacteria what is the molecule that donates hydrogen for photosynthesis?
A: Introduction - Sulfur-oxidizing bacteria are prevalent. Thiobacillus thiooxidans is a…
Step by step
Solved in 2 steps with 2 images
- HBs Antibody Principle: This test uses the “sandwich principle”, a slide phase colloidal gold enhanced immunoassay technique for determination of HBs antibody in human serum or plasma. The nitrocellulose membrane was immobilized with HBs antigen on the test band region and anti-HBsAg antibody on the control band region. During the assay, the specimen is allowed to react with the colored conjugate (HBs Ag colloid gold conjugate); the mixture then migrates chromatographically on the membrane by the capillary action. For a positive result, a color band with the specific antibody-HBsAg complex will form on the membrane. Absence of this colored band in the test band region suggests a negative result. To serve as a procedural control, a colored band at control region always appears in the test area. The reagent contains scavenge antibodies to reduce nonspecific reactivity in human serum or plasma specimens. The sensitivity of the test is 10mIU. HBs Antigen Principle:…Counterstaining with Hematoxylin and Eosin is an important step in which of the following techniques? ELISA Gel Electrophoresis IHC Western blottingCentrifugation is the first step in most fractionations, but it separates only components that differ greatly in size. Compare and contrast very high, high, medium, and low-speed centrifugations of supernatant mixtures. Define the following: SDS Polyacrylamide-Gel Electrophoresis and Immunoprecipitation
- The total number of cells in a culture is counted using the trypan blue exclusion assay and is found to be 6.8 x 106 cells/ml. Each well in a 6 well plate requires 2 x 105 cells. How should the solution be diluted so that 1ml can be added to each well?This is an SDS-PAGE of an antibody purification sample with IgG seperated from a Bovine Calf serum. Would you be able to describe the bands that are appearing and why it appeared on the gel this way? As well, a western blot was done after the SDS-PAGE and the bands that appeared were at 50 kDa and 150 kDa for the IgG and two elution lanes. May you please explain?Conjugated enzymes are a key component of ELISA. To what are these enzymes conjugated, and why is that important? To the antigen so the enzymes are only present when the molecule of interest is also present To the plastic of the reaction well so the enzymes can concentrate the molecule of interest To the capture antibody so the enzymes are only present when the molecule of interest is also present To the detection antibody so the enzymes are only present when the molecule of interest is also present
- Monospot: inconclusive Heterophile antibody titer of 1:56 Differential tests: Guinea pig kidney titer of 1:56 Beef red blood cell titer of 0 How would you interpret the above results? What other confirmatory test can be utilized to resolve doubtful?What are the advantages and disadvantages of agglutinationtests versus fluorescent antibody assays? How are the latter usedto identify specific cells in complex mixtures, such as blood?In Western Blots and ELISAS, the following molecules are used: Tween-20 SDS Goat anti-rabbit IgG (whole molecule) – HRP Rabbit anti-egg albumin Bovine IgG Rabbit anti-bovine IgG – HRP Egg albumin (globular protein – what is Molecular weight?) Milk protein (globular protein – what is Molecular weight?) TMB Beta-mercaptoethanol Methanol HCl May you explain the reasons why they are used?
- A.Why do you plate the cells from the viable count on LB agar without ampicillin? B.If you observe 100 colonies on your 1/100 plate, how many colonies do you expect if everything works perfectly on your 1/1000 plate?Briefly explain why this is ELISA referred to as colorometric assay.Explain why a semi-log plot should be used for determining antigen concentration by ELISA.