The pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L = L1 + EAg/AgCI + Easy= constants L1 = - 0.0592 log a2 a1 = activity of analyte solution a2 = activity of internal solution How will measured pH value be affected vs “real” pH if the temperature of the sample is 30C when pH was measured ? a.measured pH is lower than real pH b.impact can not be determined c.measured pH is higher than real pH d.measured pH is same as real pH

The pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L = L1 + EAg/AgCI + Easy= constants L1 = - 0.0592 log a2 a1 = activity of analyte solution a2 = activity of internal solution How will measured pH value be affected vs “real” pH if the temperature of the sample is 30C when pH was measured ? a.measured pH is lower than real pH b.impact can not be determined c.measured pH is higher than real pH d.measured pH is same as real pH

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter19: Biomedical Laboratory Instrumentation

Section: Chapter Questions

Problem 7P

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The pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L = L1 + EAg/AgCI + Easy= constants L1 = - 0.0592 log a2 a1 = activity of analyte solution a2 = activity of internal solution How will measured pH value be affected vs “real” pH if HCl in pH electrode, became 0.15M instead of 0.1M ? a.impact can not be determined b.measured pH is higher than "real" pH. c.measured pH is lower than "real" pH. d.measured pH is same as "real" pH.
Table 2. Volume of BSA, protein content, and absorbance readings of reference solutions Solution Volume of BSA standard solution (μL) Protein content(μg/mL) Absorbance value At 595 nm 1 0 0 0 2 10 1 0.022 3 30 3 0.065 4 50 5 0.106 5 70 7 0.178 6 100 10 0.299 7 120 12 0.380 Make a graph by plotting the absorbance values versus the BSA protein content (in μg) for theseven reference solutions. When constructing the graph, be…
When a SBIA021 student measured the pH of 1.0M HCI, the student obtained a pH meter and failed to neither check the condition nor the manner of usage of the instrument. The pH meter reading timed out (Did not give a reading) Which of the following problem explanations is most correct? A)The sample in the glass beaker was being swirled vigourously while the electrode was submerged in it for a reading. B)The electrode got submerged much deeper into the solution than it should have. C)All of the above are correct problem explanations O D)The electrode had long direct contact with the environment and the glass membrane got dry.
3 mL of a 45 mM stock solution of a substrate is added to 8 mL of water. Calculate the following values. The substrate molecular weight is 125 g/mol. Calculate the following values: Substrate Volume in mL Dilution Factor Substrate number of moles Substrate concentration for the diluted solution in mM Substrate concentration for the diluted solution in mole/L Substrate concentration for the diluted solution in mg/mL
Is there a difference between pH measurements using the pH meter and the pH paper? Compare the initial pH and the final pH of each sample. What was the most effective buffer? What was the least effective? Solución pH inicial pH final Diferencia water 7.45 2.48 0.1 M NaCl 7.28 2.03 milk 6.78 6.45 buffer de fosfato 8.76 7.78 **** Calculate the difference (subtract) the initial pH - final pH values to identify the effectiveness of each solution as a buffer. Values with little difference mean that a less drastic change occurred and are therefore more effective. On the contrary, values by much difference reflect drastic changes in pH and are less effective. What do buffers do and why are they important in biological systems?
Two compounds have Rf values of 0.50 and 0.61, respectively. The solvent front on a plate is developed to 10 cm. How far apart in centimeters are the spots for the two compounds?
The pH of a solution containing 20 mL of 0.120 N NaOH and 35 mL of 0.120 N NaOH is 1.49. True or False?
You have a 6 M solution of stock A. You do the following serial dilutions: Add 1 ml of stock A to tube B. Add 9 ml H2O. Mix.Then take 1 ml from tube B and put it into tube C. Add 19 ml H2O. Mix. Then put 5 ml from tube C into tube D. Add 5 ml H2O. A. what is the final (cumulative) dilution of stock A (dilution factor)? B. What is the final concentration (include units) after all these dilutions?
How much of the enzyme proteinase k (solute) is required to make 250ml of a solution with a concentration of .01mg/ml? (Weight/volume ratio)
A calibration curve for the absorbance of an unknown is shown below. What would be the concentration of a sample with absorbance of 0.45. Give the answer with the correct unit.
Given a BSA stock solution of 4mg/mL how would you prepare a 5 mL sample with a final concentration of 1 mg/mL?
What is the pH of the following buffer mixtures? (a) 100 mL 1 M acetic acid plus 100 mL 0.5 M sodium acetate (b) 250 mL 0.3 M phosphoric acid plus 250 mL 0.8 M KH2PO4