Your animal cell culture laboratory suddenly suffered from a bacterial contamination. Several components like vitamins, yeast hydrolysate, and fetal calf serum are temperature sensitive and must be filtered through a bacteriological filter to add to the autoclaved animal cell culture media. After careful considerations, you found that there are no issues with the autoclaving, but there is something wrong with the filter sterilization process, and changing new batches of filter did not remove the bacterial contamination. Which of the following rationale best describes the probable cause? Group of answer choices A. The media is probably contaminated with E. coli, which passed through the 0.2-micron bacteriological filter. B. The media is probably contaminated with Mycoplasma, due to the lack of cell walls they are the only bacteria that can pass through the 0.2-micron bacteriological filter. C. All of the other answer choices could be correct D. The media is probably contaminated with yeast from the contaminated yeast hydrolysate and not bacteria, which passed through the bacteriological filter.

Your animal cell culture laboratory suddenly suffered from a bacterial contamination. Several components like vitamins, yeast hydrolysate, and fetal calf serum are temperature sensitive and must be filtered through a bacteriological filter to add to the autoclaved animal cell culture media. After careful considerations, you found that there are no issues with the autoclaving, but there is something wrong with the filter sterilization process, and changing new batches of filter did not remove the bacterial contamination. Which of the following rationale best describes the probable cause? Group of answer choices A. The media is probably contaminated with E. coli, which passed through the 0.2-micron bacteriological filter. B. The media is probably contaminated with Mycoplasma, due to the lack of cell walls they are the only bacteria that can pass through the 0.2-micron bacteriological filter. C. All of the other answer choices could be correct D. The media is probably contaminated with yeast from the contaminated yeast hydrolysate and not bacteria, which passed through the bacteriological filter.

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.4: The Gram Stain

Problem 2RQ

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