Your are working in a biodiesel production plant and are trying to optimize the production of biodiesel (Fatty Acid Methyl Esters). The enzyme lipase catalyzes a key step in this reaction (See below). You are tasked with mutating a recombinant lipase to improve the efficiency of the reaction to improve yields of the Fatty Acid Methyl Esters. Which kinetic parameters would you calculate to determine the efficiency of the mutate enzymes. Briefly explain the design of an experiment to calculate these parameters. LIPASE + H,C-OH Lipase production Vegetable oil Methanol Biodiesel Transesterification он Lipase -OH OH он Triglyceride Fatty acids Glycerol

Your are working in a biodiesel production plant and are trying to optimize the production of biodiesel (Fatty Acid Methyl Esters). The enzyme lipase catalyzes a key step in this reaction (See below). You are tasked with mutating a recombinant lipase to improve the efficiency of the reaction to improve yields of the Fatty Acid Methyl Esters. Which kinetic parameters would you calculate to determine the efficiency of the mutate enzymes. Briefly explain the design of an experiment to calculate these parameters. LIPASE + H,C-OH Lipase production Vegetable oil Methanol Biodiesel Transesterification он Lipase -OH OH он Triglyceride Fatty acids Glycerol

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter4: Cells

Section: Chapter Questions

Problem 1ITD

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Similar questions

In the Avery experiment, mice were killed if they injected with S strain cells. Furthermore, incubation with extract from dead S strain cells could transform non pathogenic cells into pathogenic cells. This effect of S strain extract was eliminated if S strain extract is treated with what enzyme before incubation with non-pathogenic cells? protease DNAse RNAse Lipase
Consider a hypothetical enzyme that is COMPLETELY deactivated when a Valine (Val) residue buried in the core of the enzyme is mutated to Glutamate (Glu). In one or two sentences max, explain the MOST LIKELY reason for this occurrence.
For the following problem, indicate whether enzyme is repressed (-) or produced (+). Please show all possible outcomes.
What features distinguish enzymes that undergo allosteric control from those that obey Michaelis-Menten equations? Give 2 differences
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Is amino acid activation energetically favored? Why or why not?
. In Figure 6-11,a. in view of the position of HPA oxidase earlier in thepathway compared to that of HA oxidase, would youexpect people with tyrosinosis to show symptoms ofalkaptonuria?b. if a double mutant could be found, would you expecttyrosinosis to be epistatic to alkaptonuria?
Briefly describe the induced-fit conformational change when hexokinase binds its substrate.
The enzyme mutase which is important for the synthetic of tyrosine and phenylalanine in saccharomyces cerevisiae has been studied as an example of an allosteric enzyme. Tyrosine acts as a negative effector for this enzyme. What effects would you see on the action of the enzyme were you to increase the concentration of tyrosine? The chorismate mutase would shift to its R conformation The curve showing the kinetics or chorismate mutase would shift to the right The curve showing the kinetics of chorismate mutase would shift to the left The chorismate mutase would become saturated more rapidly
Biotransformation. Explain the process of enzyme induction. What are the benefits or down-falls of this process.
An uncatalyzed reaction has keq=50. in the presence of an appropriate enzyme.the forward rate of the reaction increased by 20-fold.what is the equilibrium constant in the presence of the enzyme?
Please asnwer the following question: Suppose you specifically want to measure the amount of beta-glucose present in a sample. You have an enzyme that reacts specifically with the beta form of the sugar,and produces a signal that is easy to measure. However, every time you try to measure the amount of beta-glucose, mutarotation occurs and the alpha form of the sugar is converted into beta!! Can you suggest a simple way of overcoming this problem so you could measure only the beta form of the sugar? HINT: Is there a way you can prevent mutarotation?