You’ve made your construct and placed it into E. coli!  Congratulations, you have made a transgenic organism.  Your investors will want to know about quality control. How will you check that the correct piece of DNA is in your vector? How will you check to make sure the gene is transcribed? How will you check to make sure that the GasP protein is made in E. coli?

Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter14: Biotechnology And Society
Section: Chapter Questions
Problem 7QP
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You’ve made your construct and placed it into E. coli!  Congratulations, you have made a transgenic organism.  Your investors will want to know about quality control.

  1. How will you check that the correct piece of DNA is in your vector?
  2. How will you check to make sure the gene is transcribed?
  3. How will you check to make sure that the GasP protein is made in E. coli?
  4. Your investors are concerned that the GasP protein might not be sufficiently produced under normal laboratory conditions. They suggest controlling the transcription of the gasP gene using a chemical that will “trigger” its transcription.
    1. What type of promoter could be used?
    2. What chemical will you use to control transcription?
    3. How does this method of control work?

 

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