You just graduated from college and started working at a biotech startup called Scrofabulous. Your first job assignment is to clone the pig gene for the hormone prolactin. Assume that the pig gene for prolactin has not yet been isolated, sequenced, or mapped; What would be the most useful and economical first step to go about identifying and cloning the pig gene for prolactin? use the amino acid sequence of mouse prolactin to design a pair of degenerate oligonucleotide PCR primers to PCR-amplify the pig prolactin gene. RNAseq the pituitary gland of the pig, the most abundant gene is likely to to be prolactin Conduct a proteome search for peptides that match parts of mouse prolactin protein Sequence the pig genome, then translate the genome to find the gene predicted to encode for prolactin O Crystalize the mouse prolactin protein and use Google's DeepMind Al to find the closest amino acid sequence in the pig proteome

Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter14: Biotechnology And Society
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You just graduated from college and started working at a biotech startup called Scrofabulous. Your
first job assignment is to clone the pig gene for the hormone prolactin. Assume that the pig gene
for prolactin has not yet been isolated, sequenced, or mapped; What would be the most useful and
economical first step to go about identifying and cloning the pig gene for prolactin?
use the amino acid sequence of mouse prolactin to design a pair of degenerate oligonucleotide PCR primers
to PCR-amplify the pig prolactin gene.
RNAseq the pituitary gland of the pig, the most abundant gene is likely to to be prolactin
Conduct a proteome search for peptides that match parts of mouse prolactin protein
Sequence the pig genome, then translate the genome to find the gene predicted to encode for prolactin
Crystalize the mouse prolactin protein and use Google's DeepMind Al to find the closest amino acid
sequence in the pig proteome
Transcribed Image Text:You just graduated from college and started working at a biotech startup called Scrofabulous. Your first job assignment is to clone the pig gene for the hormone prolactin. Assume that the pig gene for prolactin has not yet been isolated, sequenced, or mapped; What would be the most useful and economical first step to go about identifying and cloning the pig gene for prolactin? use the amino acid sequence of mouse prolactin to design a pair of degenerate oligonucleotide PCR primers to PCR-amplify the pig prolactin gene. RNAseq the pituitary gland of the pig, the most abundant gene is likely to to be prolactin Conduct a proteome search for peptides that match parts of mouse prolactin protein Sequence the pig genome, then translate the genome to find the gene predicted to encode for prolactin Crystalize the mouse prolactin protein and use Google's DeepMind Al to find the closest amino acid sequence in the pig proteome
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