Bacteriology

Introduction: Biochemical tests are used to identify bacteria species based on their differences in their biochemical activities of different bacteria. To differentiate one bacteria from another, each species of bacteria has a well-defined set of metabolic activities. Bacteria can be differentiated in many ways. They can be based on structural differences or metabolic differences. Structurally, the Gram stain is the most fundamental technique in identifying bacterial species and was developed by

Microbial Unknowns Objective: In the past couple of weeks, our class received a single broth that contained two species of bacterium. One bacterium was gram positive and one bacterium was gram negative. We were required to use our techniques and knowledge we learned in class to separate and identify the two species we were given in our broths. Methodology: Day One: On the first day of our Biochemical Unknown Lab we were given a tube that contained broth media which held two different types of

Table 1 shows the steps and color results after each reagent was added to bacterial smear slide. Before any reagent was added to the heat fixed bacterial slides unknown bacteria was colorless. The primary stains reagent, Crystal Violet was added to the slide the unknown bacteria color was purple. Then, Iodine mordant the color of cells still remained purple. After adding the decolorizer alcohol gram + would remain purple and the purple color from the gram- would be removed and it would become colorless

The unknown bacteria B22 contained an opaque white, circular colony, along with an irregular margin on the initial inoculated agar plate. The broth characteristics included growth with the presence of a flocculent and all around cloudiness; however, the slant characteristics included just a spreading edge. After the bacterium identified to be gram positive, a blood agar plate identified the bacteria to have gamma hemolysis capabilities; additionally, the mannitol salt agar identified the bacterium

Gram Stain In 1884, a Danish pathologist, Christian Gram, discovered a method of staining bacteria with pararosaniline dyes. By using two different dyes, he discovered that bacteria fall into two different groups. The first group, gram positive, retains the primary color, crystal violet. The second group, gram negative, when washed in a decolorizing solution, becomes colorless and takes on the color of the counterstain. The reaction of the Gram-stain in a bacteria is determined by the biochemical

Title Page  Introduction The purpose of this lab is to use several techniques to determine the identity of an unknown bacterium. Many of these tests involve the differences between Gram-positive and Gram-negative bacteria. Gram-positive bacteria have a thick peptidoglycan wall while Gram-negative bacteria have a much thinner peptidoglycan wall. This leads to a number of differences in what media each kind of bacteria can grow on and their physical properties. Techniques used in this experiment

Proteus vulgaris #12 The importance of identification of a certain microorganisms can range between a life threatening diseases to a creation of certain antibiotic. Understanding the principals of living microbes and identifying my unknown bacteria through numerous biochemical and metabolism tests, with the outmost confidence, Proteus vulgaris had the precise qualifications. The point of this report is to further explore the identification of my unknown bacteria by revealing the results of the

Matthew Crane Lab Section: 13151 Comparative Analysis of Antibiotic Growth Suppression of Staphylococcus Aureus Introduction Staphylococcus Aureus is a gram negative bacterium that may be pathogenic or exist as a commensal organism in humans. Unevolved strains are susceptible to nearly all antibiotics (Chambers & DeLeo, 2009). Among the many antibiotic options, penicillin is particularly effective against S. Aureus. This is due to the mechanism of action by which penicillin replaces key structures

In this experiment individuals were given a plate with two unknown specimens. Each group completed a gram stain followed by a series of other test in order to identify the unknown bacteria. Each student conducted separate testing’s on one unknown in order to specifically identify the strain. Materials and Methods First and foremost the gram stain is conducted to differentiate gram positive bacteria from gram negative bacteria due to their cell structure. The thick layer of peptidoglycan

Abstract The identification of bacteria is a crucial procedure in the field of biology. Identification can be done through a series of tests run on different forms of media that detect the physical and biochemical properties of the cells. In this experiment, a sample containing two unknown species of bacteria was run through fourteen tests to determine morphology, fermentation properties, reducing and oxidative properties, and other various characteristics of each isolated unknown. It was concluded