Bacteriology

The objective of this experiment was to identify two unknown bacteria from a mixed culture. Which was done by using the aseptic technique which was very important to avoid any contamination and keeping the workspace clean while culturing bacteria for different tests. To start, I chose a tube which had a solution of mixed culture. I used the flame to sterilize the inoculating loop and dipped it into the tube and streaked for isolation on 2 TSA plates and placed them in an incubator at 37 for 24 hours

When I first did my streak plating I could only find two microbes and I was panicked. I thought the third one was a fastidious bacterium. It turned out that the third one was there all along; the growth was just too vague. I was able find it the very next day. I gram stained my bacteria and I found out I had one gram positive and two gram negatives. I called my gram positive U1 and my gram negative U2 and U3. My gram positive was rods so I was suspected it to be a Bacillus species. I waited for

In order to isolate fecal coliforms, Eosin Methylene Blue agar, which contains peptone, lactose, sucrose, and the dyes eosin Y and methylene blue, is used. These sugars provided encourage growth of fecal coliforms while the dyes inhibit growth of Gram-positive organisms. As a result, EMB agar allows the inoculation of Escherichia coli, Enterobacter aerogenes, Salmonella typhimurium, and Enterococcus faecalis. To identify E. coli strains on EMB agar, the cultivated bacteria must appear green, black

The Staphylococci species are abundant in our environment and in the normal flora of the human body. On our bodies, they are found primarily on the skin and upper respiratory tract, as well as the anterior nares and pharyngeal surfaces. Staphylococci are Gram-positive cocci, typically arranged in irregular clusters comparable to grapes. Facultatively anaerobic organisms with the ability to grow well on most nutrient media. Staphylococci are virulent because they are resistant to drying, changes in

Before starting all the biochemical tests, an inoculation procedure must happen. An unknown test tube was handed out which contained two different cultures. The unknown culture was place on a nutrient agar plate using the three-streak inoculation technique for isolation (Lab Manual, Chess 2015). After a few days of growing, the presence of two unknown bacteria had a clear visible of separation. Following the isolation streak technique, the gram-staining experiment was performed. The two bacteria

the nostrils, armpits, head, and human epithelia. Staphylococcus epidermis is a salt-loving microbe. This species can live in a harsh condition, they can live in an environment that has 10 to 15 percent of salt (Bergey’s Manual of Determinative Bacteriology, page 488). A significant whole genome adapted biofilm is shown by Staphylococcus epidermis, which can cause biofilm to growth on a plastic device that is implanted into the body. Staphylococcus epidermis only can be treated with antibiotics vancomycin

Hershey were his biological parents. While his father worked for an auto manufacturer, Alfred attended public schools in Owosso and nearby Lansing. He studied Bachelor of Science in Bacteriology from Michigan State College in 1930 and he earned his Ph.D. in chemistry from the same school in 1934. Hershey's interest in bacteriology and the biochemistry of life was clearly noticeable. His doctoral dissertation was focused on the chemistry of Brucella, the bacteria responsible for brucellosis, also known as

penicillin, which is a fungus, to make the first medicine. Alexander Fleming was very well educated to be as smart as he was. When Fleming was in college, he studied bacteriology. For college, he went to St Mary’s medical school. Fleming’s original plan was to become a surgeon but things changed and he ended up studying bacteriology. Alexander Fleming made amazing discoveries that changed medicine back when it was only used in war. His first discovery

Europe faced an era of revolutions at the wake of the nineteenth century. Long-standing empires, which had traditionally controlled most of the world –including the Spanish, Chinese, French, and Holy Roman Empires –, collapsed at the beginning of the century, allowing the British Empire to rise to power and establish dominance over a fifth of the Earth’s surface. This political revolution was coupled with the industrial revolution. New manufacturing processes called for large industrial factories

people do obtain bacterial infections at times, majority of the time the human body prevents bacterial infections from affecting the body. During this researching period, Fleming graduated St. Mary’s Medical School once again with a Bachelor of Bacteriology at the same time receiving the gold medal for the top student (Maurois, 1956). In 1914 Fleming concluded his research under Almroth Wright’s guidance and unknowingly continued his journey on to becoming the man with the answer to the biggest human