Bacteriology

In the world of microbiology it is vitally important to be able to discern the identities of microorganisms. Not only is it important in a lab setting but as well as in healthcare in general. Properly identify what strain of bacteria a person has will aid in the proper medicine and dose given. Throughout the semester we have learned about different types of bacteria and certain test that can clearly identify them. The purpose of this lab report is to identify a Gram-positive or Gram-negative bacterium

3.1. Screening for Bi NPs producing microorganism The obtained results of the screening step revealed that one sample [taken from salty marsh land around Shahr-e Babak, Kerman, Iran] contained a bacterial strain (Fig. 1a) capable to reduce Bi3+ ions into Bi0 which was evident from color change from yellowish into dark brown (Fig. 1b). Similar results were observed when the selected isolate was cultivated in the culture flask including Bi3+ ions compared to that of culture media without Bi3+ ions

First a thin smear of bacteria, placed on a clean slide, covered with crystal violet stain for 1 minute. Next, completion of a wash and then an iodine solution is added to the smear to enhance staining for 1 minute. Being rinsed again in water and then 95% ethanol for one minute. Washing the violet stain off will differentiate between the two types of bacteria. If the bacteria retains the violet color, it means they are gram positive, while the others without strain are gram negative. The cell

For the first day, I received my unknown bacteria number 110 and I stared to observe that tube. The tube has normal color, creamy, and little bit cloudy. First, I made two new slants to get the strong and alive bacteria for further text. One tube was incubated at 250C and other at 370C. Next, I started to do my Gram Stain. After I finished the smear, heat fix, and cover the slide with all chemical, I saw the pink color stick onto the slide. I knew that this bacterium is gram negative. Then, I looked

All of the T-streak showed growth however, not all of the bacteria formed colonies. Pseudomonas aeruginosa had no colonies therefore, the colony morphology were not applicable. The error could have been to not spreading the bacteria enough using the T-streak method. P.aeruginosa, Enterobacter aerogenes, Escherichia coli and, Klebsiella pneumoniae all tested as gram negative. The lab manual confirmed the experiments findings to be correct and each one were pink colored on the slide under a microscope

Microorganisms have the ability to live everywhere and can survive when put together with another not of its kind. Expectations for this experiment would to successfully obtain credible information behind the mixed unknown and be able to isolate both the gram-negative and gram-positive bacterium. In order to obtain the correct test results and to ensure the right gram mixed unknown is identified there had to be a series of eight biochemical tests performed on the gram-negative bacterium and five

1. What type of agar is used for the growth of these bacteria? The identification of N. meningitides, H. influenza and S. pneumonia can be done through the cytology of CSF and through the specifics of colony morphology on blood or chocolate agar. Is this agar chemically defined or complex? The agar is chemically defined 2. What type of staining technique would you use on the he sample to aid in identification of the bacteria? The staining technique used to identifying would be a latex agglutination

The first test done for Klebsiella pneumoniae (the gram stain) indicated that this bacteria was rod shaped and therefore, using the dichotomous key the family of bacteria that were ruled out consisted of the genera Neisseria and Rodospirillum. The next tests that were vital to the identification process were the oxygen requirements and glucose fermentation tests. The test results gave us the confirmed family of our species which was Enterobacteriaceae. The negative oxidase test performed confirmed

To find the first unknown bacteria, the microbiology student started by inoculating a sample on a slide to stain with Gram’s stain; resulting a gram positive cocci. He continued by looking at the dichotomous key in the Lab Manual, afterwards doing a catalase test on an inoculated slide to differentiate it from being either a Streptococcus or a Staphylococcus (2). He then put the hydrogen peroxide on the inoculated slide, it immediately reacts, resulting on an excessive bubbling reaction, concluding

Purpose: The purpose of a Gram Stain procedure is to distinguish bacteria into two fundamental groups of cells. There are the Gram positive cells and the Gram negative cells. By staining the cells either violet or red, we can see the two types and their arrangements on the smear. Theory and Background: 1. After swabbing some microbes from my cheek, I spread the bacterial smear on the slide. In order to make them stick to the slide, I hold it near a heat source so they can attach. Then I flood