Bacteriology

The first unknown test tube P found to be a gram positive bacteria. The biochemical that I performed that were positive were catalase and blood agar. Catalase has the presence of an enzyme in the test isolated detected using hydrogen peroxide. Bacteria possess catalase with a small number of bacteria; isolation is added to hydrogen peroxide when bubbles occur. When the bubbles occurred on gram positive bacterium it was a conformation of being positive. Also, blood agar plates is useful for cultivating

In category IV, agar plates were used containing differential and selective aspects to determine the unknown bacterium. The first media test was the EMB. EMB separates fecal coliforms that are produced through carbohydrates that are fermentable, such as sucrose and lactose. The different fecal coliforms represent the differential factor of this test, while the selective factor selects against gram positive organisms. Lactose fermenters appear purple, pink, blue, and black colonies, while non-lactose

Staphylococcus aureus embraces many characteristics which are derived by a variety of test to identify if someone has this certain bacteria. This specific species is recognized by the anaerobic fermentation, beta hemolytic halo, coagulase positive testing, the Kirby-Bauer disk diffusion test resistance, clumps of gram positive cocci that is identified through the microscope, and the a positive catalase test (Kiser 67-87). First in the series of test was the mannitol salt agar MSA plates, which contains

NAME: Zachary R Short Unknown Number: 118 Points: _________ (50 Possible) Title: Identifying a Gram Positive and Gram Negative bacteria from a mixed broth Abstract: I inoculated a T-Soy agar with bacteria number 118, for this I used a streak isolation method. Next, in order to distinguish between Gram positive and Gram negative I used a streak isolation technique on a CNA plate, then performed the same exact procedure on a MacConkey plate. The results from the CNA plate showed the

The first patient, 18 year old female presented with symptoms of high fever, vomiting, diarrhoea, myalgia, hypotension and signs of septic shock. In order to identify possible bacteria responsible for these symptoms, a vaginal swab was sampled, incubated on agar overnight and stored at 4C, after which they were examined throughout a series of microbiological tests. The initial culturing of the sample on nutrient agar indicated two organisms were present, and hence these were analysed individually

MANIPULATION OF BACTERIA INTRODUCTION: In this experiment that we performed, there were many methods that were used to help us manipulate and identify the bacteria E.coli on a MacConkey agar plate. The first part of the experiment involved the methods of manipulating, identifying and counting the bacteria and the second part was to find out whether the bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is

Comparing the cellular morphology of table 2 to their respective pure culture in table 3. B. cereus presented with a white colony, round colony morphology, smooth margins and flat elevation in both pure culture and isolated culture; E. coli presented with a Yellow colony, round colony morphology, smooth margins, and flat elevation; S. aureus presented with a White colony; round colony morphology, smooth margins, and raised elevation. In comparison of the cellular morphology of each bacterium in

Gram Negative Salmonella Typhimurium (#16) Lab Report Heather A. Rogers Nov. 9 2016 Biol 2460 University of Texas at Arlington  Abstract This lab experiment was done for the purpose of learning how to determine a gram negative bacterium based on multiple tests learned throughout the semester. My gram negative unknown bacterium given to me was Salmonella typhimurium based off of the following tests; Triple Sugar Iron Agar (TSIA), Sulfate Indole Motility (SIM), Methyl Red (MR), Voges-Proskaur (VP)

The Gram-negative bacterium for Unknown 13B was Salmonella typhimurium. Unknown 13B had similar reactivity patterns to S. typhimurium. A comparison of Unknown 13B and S. typhimurium can be compared in Table 2. The Catalase test and the Methyl Red test for Unknown 13B and S. typhimurium both obtained positive results (Table 2). Negative results were acquired through the Indole test for Unknown 13B and S. typhimurium (Table 2). For the Lactose Ferment and the Sucrose Ferment test, negative reactions

The goal of the streptococcus lab was to isolate and characterize the type of streptococcus microorganism that may be located in the back of the one’s throat (the source of the specimen). In order to isolate a specific type of organism, a sample is collected by doing a throat swap. The sample was streak on a Columbia Nalididxic Acid agar (CNA) plate by using the three way method. The CNA was used as a selective media for colistin and nalidixix acid but also used for differential reactions for hemolytic