Microscope

parts of the confocal microscope. It will follow by more information about fluorescence microscopy which is used for study fixed and living cells because of its versatility, specificity, and high sensitivity. Introduction: The Confocal Microscope is a type of microscope that is being developed for obtaining high-resolution images and 3D reconstruction. The images from the confocal are far better then other microscopes; however, not quite as good as fluorescent microscopes. The confocal shows the

MICROBIAL SURVEY, SMEAR PREPARATION, AND SIMPLE STAIN Instructional Objectives 1. Define Roccal = green, liquid disinfectant. Pathogen = an agent which causes disease. Wet Mount Slide = a microscope slide of a liquid specimen covered with a cover glass. Yeast = a single celled fungi. Budding = a true characteristic method of asexual reproduction among yeasts where budding of a new cell from a parent cell can be observed. Mold = multicellular masses of filamentous fungal growth. Hyphae

LITERATURE REVIEW The microscope has been one of the greatest contributions to scientific study known to mankind and like a hammer is found in every carpenter 's toolbox, one would find a microscope in a scientist 's toolbox. A microscope is used to view matter not obvious with 20/20 vision and can magnify objects as small as the smallest atom. The nomenclature for microscope derives from two words, mikros and skopein which means seeing small. The history of the microscope, which is comprised

the microscope. One of the first to use a microscope to observe microorganisms was Robert Hooke, the English biologist who observed algae and fungi in the 1660s. In the 1670s, “Anton van Leeuwenhoek, a Dutch merchant, constructed a number of simple microscopes and observed details of numerous forms of protozoa, fungi, and bacteria” (Introduction to Microscopes, n.d.). During the 1700s, microscopes were used to further explore on the microbial world, and by the late 1800s, the light microscope had

dihydroethidium (DHE) as an indicator.Serial, coronal frozen-sections (5µm thick) were incubated with DHE (10 μmol/l) in PBS in a dark humidified chamber (37°C, 30 min). Five fields from each coronal section were randomly selected by Nikon A1 laser confocal microscope. Images were set by 20× objective lens at 1024×1024 pixels. ROS levels were detected by the fluorescence density. Western blot The hippocampi were isolated from right brain for Western blot. Western blotting was performed using the standard method

On August 11, 1926 a Jewish couple Lazar and Bella, gave birth to their son Aaron Klug. By the time Aaron was two, him and his family left Lithuania and moved to South Africa. His father was a trained saddler and Aaron often helped his father rear and sell cattle during his teenage years. When it came to education, Klug had a traditional Jewish education and secular schooling. In primary school, Klug read Microbe Hunter by Paul de Kruif which inspired him to study medicine as well as microbiology

Exercise 1: Identifying Parts of a Compound Light Microscope Question A. Study and label the microscope parts in Figure 3. Microscope Parts Microscope Parts A EYEPIECE G MIRROR/ILLUMINATOR B TUBE H BASE C REVOLVING NOSEPIECE/TURRET I LONGITUDINAL CONTROL KNOB D OBJECTIVE LENS J TRANSVERSE CONTROL KNOB E STAGE K FINE ADJUSTMENT F DIAPHRAIGM L CASE ADJUSTMENT Microscopy Use and Function Exercise 2: Illuminated Pocket Microscope Questions A. Draw the image of the root

fibers tested were part of a multi-fiber fabric with the following arrangement: Acetate Cotton Nylon Silk Viscose (Rayon) Wool The first lab activity was to observe and record the characteristics of each of these fibers through a stain test, a microscope test, a solvent test, and a burn test. The second lab activity was to observe the characteristics of an unknown fiber thread and to identify the fiber by comparing its characteristics to the data from the first activity. The burn test is used to

Distinguish between the resolving power and magnifying power of a microscope lens system. a. resolving power: b. magnifying power: 8. In addition to bright field microscopy list two other variations on the optical microscope. a. b. 9. Why is it necessary to use immersion oil when using the 1000X objective lens to observe a specimen? 10.

familiar with the lighted microscope, how to prepare a wet mount, and understand taking measurements in the metric system and its conversions.  Materials & Methods Materials: Part A: * Computer for videos Part B: * Tape measure with centimeters * Scale that measures grams * Thermometer that shows Celsius * Campbell Biology Book * 1 piece Orange Starburst Methods: Part A: Combined Lighted Microscope 1. Watch video on microscope 2. Answer the provided