Molecular biology

successful genotypic and phenotypic mutations, which will display fluorescent under ultra-violent lights or show signs to being resistant to ampicillin. This experiment was primarily for the purpose of growing E. Coli bacteria. Introduction In molecular biology, transformation is the genetic alteration of a cell resulting from the uptake and expression of foreign genetic material (DNA) [1]. Bacteria evolve rapidly not only by mutation

1. Green Fluorescent Protein (GFP) GFP is a widely used tool in the field of Molecular biology and Cell biology. It involves emission of fluorescence under Ultra Violet light which allows for direct investigation into the inner working of cells. Green Fluorescent Protein was first isolated from the jelly fish Aequorea victoria by Osamu Shimomura (Shimomura et al., 1962). Since its discovery, it has become useful in the field of science. The GFP chromophore is formed from tri peptide in the primary

Shaddi Martinez Ms. Herrera LSC 348 Lab 12/3/16 pGLO Transformation: A Look into Our Future with DNA and Genetics Abstract This lab investigated the central dogma of molecular biology by studying the expression of jellyfish Green Fluorescent Protein in E. coli (Mardigan, 2011). After transforming E. coli with the GFP gene under the arabinose operon promoter, GFP expression was analyzed with and without arabinose. Induction was seen only in media with arabinose. To further study GFP protein and structure

to the blood, urine and feces. Question #2 Plasmid DNA is an autonomously replicating circular DNA molecule that is only about 1,000-20,000 base pairs in size and is separate from bacterial chromosomes. Plasmids are a significant part of molecular biology because they are small enough to be cloned or modified, they endure self-replication, they are stable over long periods of time and they have a diverse range of gene expression. Having these characteristics allows plasmids to introduce genes

I am writing with regard to Research Assistant, Technical Staff or Technician (Stem Cell Biology and Regenerative Medicine) advertised on website. Please find attached my Curriculum Vita, which shows I have the necessary qualifications and experience in the workplace. My degree course has prepared me well for this position. During my dissertation, I was required to do a lot of independent research, which required self-motivation, and the ability to organize and work on a long-term project

TRANSFORMATIONS INTRODUCTION Green Fluorescent Protein (GFP) is a fluorescent protein found in jellyfish that causes organisms to fluoresce. This protein was the first fluorescent protein to be discovered and has been highly useful in a broad range of cell biology disciplines; because of it’s highly useful reporter genes and the ability to use multicolor protein tracking in living cells it has been useful in many scientific experiments such as E. Coli transformations. There have been many other fluorescent

In order to conduct this experiment, three types of evolutionary trees are built in order to compare similarities and differences between the organisms. The first tree is an organization of the 11 organisms given. The http://www.boldsystems.org/ website is used because it provides the organisms’ phylum, class and order. The 11 organisms from the fungi kingdom are in the ascomycota, basidiomycota, zygomycota and chytridiomycota(outgroup) taxonomic groups. To create the classical tree, compile information

tagged with fluorescent markers are extremely versatile tools in cell and molecular biology research. A fluorescent marker is, as the name suggests, a molecule that emits light of a particular wavelength following exposure to a photons of a shorter wavelength, for example a laser beam. If a fluorescent marker is a reasonable size and chemically stable enough to be attached to a protein, it is potentially useful to molecular biologists. In many cases, the proteins in question are monoclonal

drugs interact with the same gene product. The literature of biological studies contains most of the available information about gene-drug relationships. Identifying drug-drug interactions is an important and challenging problem in computational biology and healthcare research. One approach that has been tried and has been successful is text mining. It provides an attractive solution for identifying gene-drug relationships and aggregating them to predict novel DDIs. There are accurate, structured

at Reed College. Before joining Reed College, I was a Senior Research Associate at the CUNY Institute for Macromolecular Assemblies and an Adjunct Assistant Professor at City College of New York. I obtained my Ph.D. in Chemistry (specializing in Molecular Biophysics) at the City University of New York (CUNY) in September of 2009. My cross-disciplinary research training in the areas of biophysics, spectroscopy, and materials science has involved projects in three different laboratories at CUNY, focusing