Restriction enzyme

PART A- Restriction Enzymes Restriction enzymes are a tool that allows us to pinpoint human identity down to single differences in our DNA. Work through the following simulation so you can see these molecular scissors in action. Find out more about restriction enzymes by viewing the animation and reading the article listed below. DolanDNALearningCenter: Restriction Enzymes http://www.dnalc.org/ddnalc/resources/restriction.html Access Excellence Classic Collection: Restriction Enzymes Background

FIFE COLLEGE Restriction Site Mapping in λ Phage DNA Daniel Richards 0612924   Table of Contents Introduction 3 Aims and Objectives 7 Methods 8 Results 13 Discussion 14 Sources of Error 15 Conclusion 16 References 17 Introduction Lambda phage, also known as enterobacteria phage λ, is a bacteriophage that infects Escherichia coli. The lambda phage has the capability to reside in the genome of its host through lysogeny or to enter a lytic phase, during which it lyses the cell to produce

Isolation, restriction digestion, and gel electrophoresis of plasmid DNA Prathyusha Gudapati, BIOL 304, spring 2015. Abstract The purpose of the experiment was to isolate plasmid DNA, followed by restriction digestion using restriction endonucleases and then visualizing the digested fragments after subjecting to gel electrophoresis. Plasmid DNA (pSP72 DNA) was isolated from Escherichia coli KAM32 (E.coli) cultures using the QIA prep miniprep kit and then subjected to restriction digestion by EcoRI

bacteria Aliivibrio Fischeri. We will be achieving this purpose by making Escherichia Coli luminescence through the use of the lux operon. In the process of understanding the genomic library of A. Fischeri bacteria, we will be creating a restriction map of the restriction sites in the plasmids containing a lux. In this study we will be working with a marine symbiont bacteria known as A. Fischeri (Bose et al., 2008). A. Fischeri is a type of organism that is gram negative and rod-shaped. The term gram

is located within the lacZ gene and contains unique sites for the Xbal & EcoRI restirction enzymes to cut and produce sticky ends for the CIH-1 gene to bind to. Furthermore, the pUC19 plasmid also contains an ampiccilin resistance gene so only transforemed E.coli are able to remain viable when spread on the agar plates that also has the addition of ampiccilin. The lacZ gene encodes the β-galactosidase enzyme which aids in indentifying the recombinant E.coli from the non recombinant cells (Coventry

In Figure 1, the photograph shows how each of our samples ran in the agarose gel. We can see different bands in different wells, meaning that the enzymes were working and that the plasmid has been cut in different patters according to the reaction. Before starting measuring the distances and doing the calculations, it is noticeable that the bands of the ladder were not very well separated. Therefore, it will not be accurate to rely on those bands to make the standard curve and determine the sample

DNA DIGESTION AND ELECTROPHORESIS In this experiment we will be doing a process called as DNA digestion or also known as restriction digest. A restriction digest is a procedure used in molecular biology to prepare DNA for analysis or other processing. It is sometimes termed DNA fragmentation, scientists Hartl and Jones describe it this way: This enzymatic technique can be used for cleaving DNA molecules at specific sites, ensuring that all DNA fragments that contain a particular sequence have the

############################## Part 1 #################################3 Antibody binding of antigens represents a critical part of the adaptive immune system’s ability to identify and eliminate invading pathogens. The antibodies are able to do so due to the binding of their antigen-binding domains to specific epitopes along an antigen’s surface. These epitopes can exist in linear, structural, and

Locating the XhoI Recognition Site on Lambda DNA Using a HindIII and XhoI double digest Haleigh Wood Abstract Restriction enzymes cut DNA at certain sites to create multiple DNA fragments. Restriction enzyme HindIII has known DNA fragment lengths and recognition sites when digesting lambda DNA, while the lambda DNA recognition site for restriction enzyme XhoI is unknown. The goal of this study is to determine the lambda recognition site of XhoI by comparing a HindIII digest and a HindIII and XhoI

INTRODUCTION At 4 pm, in a certain park, 2 skeletons were found lying side by side behind the bushes. It was concluded that the two were a couple because they were holding hands whilst the skull was facing each other. A femur, pelvic girdles, skulls, tibia, and humerus were taken from each of the skeleton and observations were made to identify their gender, race, age, and height. One is a female, and the other was a male, also the skeletons pretty much remained intact from being undisturbed however