Restriction enzyme

Often times, when new information arises in today’s society, it is very likely for facts to be altered as it translates from the original scientific study to a broad mass media article while attempting to appeal to the target audience. I recently came across a medical study regarding the effects wine causes on one’s health and weight. This article was geared towards health professionals and was based upon extensive research and studies. However, this information was slightly altered as it went to

Introduction Bacteria is used as expression host for mammalian proteins due to its rapid and simple system of expressing recombinant proteins as it has a short doubling time. E.coli is a widely used system as there is more than enough knowledge about its ‘genome sequence, physiology and genetics’ (4). E.coli is a ‘gram-negative bacterium’ (5). Bacteria has a simple genetic manipulation with rapid expression which is suitable for large-scale fermentations and makes it easier to handle in a standard

fermentation in the given time. To anaerobically break down glucose into ethanol and carbon dioxide, yeast will use the zymase enzyme which catalyses the catabolic fermentation reaction according to the induced fit model. The zymase enzyme will form weak bonds with it’s specific complementary substrate (a glucose molecule), inducing a conformational change in the shape of the enzyme and substrate. In a catabolic reaction this stresses the substrate bonds of the substrate so that they break easily, allowing

I. “Fasting is an effective and safe method of helping the body detoxify itself.” Quote by: James F. Balch, and Phyllis A. Balch. When you begin fasting, toxins are created in your body, mostly because of food and water. During your fast your body is provided resting time, most importantly, it gives your organs a rest which could potentially prolong your life. To prepare yourself for a fast you should try to eat mostly fruits, and vegetables. Intermittent fasting means fasting for certain hours of

Anorexia is a kind of dietary issue, those who have an extraordinary dread of putting on weight. They seriously restrict the measure of what they eat and can turn out to be thin. Anorexia influences both the body and the psyche. Anorexia is an intense dietary issue, especially on the off chance that it is joined by co-happening psychiatric and addictive clutters. Like bulimia anxious and other dietary problems, anorexia is a medicinal malady that can bring about irreversible wellbeing complexities

To mutagenize human Kif5A (WT), we performed PCR-mediated site-directed mutagenesis to insert our mutation into the plasmid vector (shown to the right). Next, we performed a restriction enzyme digest with Dpn1, to digest the parental plasmid.  The presence of the PCR product was confirmed by performing gel electrophoresis where the expected band of 6.8 kilobases was obtained. The remaining DNA was used in a bacterial transformation

Abstract Biofuel has become the focus of society in recent years, because the discharge of CO2 has been increasing year after year, energy crisis and environment pollution problem stunted economic growth. To solve these problems, biofuel is a good option since its low pollution and renewability. In 2010 the production of biofuels which provide 2.7% of fuels for transportation in the world reached 105 billion liters (Worldwatch Institute, 2011). The United States and Brazil were the largest producers

(positive to negative and so on). Also by size helps sort fragments as well. Small dna moves faster than larger ones do. 1. If each individual has such a small amount of DNA, how do the bands on the gel contain enough DNA to be visible?  By restriction enzymes then amplified by polymerase chain reaction to make many to millions of copies of a single fragment. 1. Many genes only have a few possible alleles. For example, humans only have a few eye colors and only four blood types. How can DNA

The significance of the enzyme Calcium/calmodulin-dependent kinase II (CaMKII) in synaptic long-term potentiation (LTP), and hence as a molecular underpin of memory, was demonstrated by Lisman et al., 1 but at the level of a single synapse, the localization of CaMKII nor its activity in the spine was previously known. Lee et al. 2 used the technique of fluorescence resonance energy transfer (FRET) and two-photon fluorescence lifetime imaging microscopy to visualize the dynamics of CaMKII in a single

quantitative description Specific sequence of amino acids recognized by their function throughout the sequence Built by scoring amino acids at each position in the alignment according to the frequency with which they occur (Gribskov M. 1987). restrictions in identifying homologues as they don’t accept mismatches Enhanced sensitivity allows deletion of poorly conserved domains or families (Sigrist C.J.A. 2002). based upon the binary response built by scoring alignments, can predict their structural