Restriction enzyme

Biology Enzymes IA Design Introduction: Enzymes are globular proteins, they are responsible for most of the chemical activities of a living organism. They act as catalysts, substances that affects the reaction of other substances without being destroyed or altered during the process. They are extremely efficient in the body system of living organisms, one enzyme may catalyse over a thousand chemical reactions every second. But there are certain conditions that need to be fulfilled in order for the

An enzyme is a biological catalyst. It speeds up a reaction by lowering the activation energy required to start the reaction. It speeds up a reaction, but remains unchanged unless certain limiting factors are introduced. It is composed of polymers of amino acids. An enzyme has an optimum pH and temperature. When an enzyme is at its optimum conditions, the rate of reaction is the fastest. In their globular structure, one or more polypeptide chains twist and fold, bringing together a small number of

Activity INTRODUCTION Enzymes are organic catalysts that spur metabolic reactions. The presence of an enzyme within a cell is essential in order for any sort of reaction to take place. All enzymes are complex proteins that act in an organism's closely controlled internal environment. In such a homeostatic environment, the temperature and the pH (concentration of hydrogen ions), remain within a fairly narrow range. Extreme variations in pH and temperature denature the enzyme by altering its chemical

A Basic Understanding of Diffusion and Osmosis The purpose of this lab was to have a basic understanding about concepts diffusion and osmosis. In this lab we had dialysis tube as a cell membrane of cell, which lets small particles pass through it and we had test tube as tightly joined surface, which doesn’t let any particles pass through it. As a result we saw that small particles like glucose and iodine diffused through dialysis tube, but large particle like starch

and measure the enzyme activity of β-galactosidase in the different concentrations of o-Nitrophenylgalactoside (ONPG) using a spectrophotometer. The spectrophotometer was also set at 420nm, a wavelength which is best for recording the absorbance values for the experiment. From the results, 0.9mM ONPG solution has the highest absorbance and 0.1mM ONPG solution has the least. Also, 0.5mM ONPG solution has the highest rate of enzyme activity and it is the most efficient as the enzyme activity of the

1.0. INTRODUCTION The primary function of the digestive system is to transfer nutrients, water, and electrolytes from the food consume into the body’s internal environment. The ingested food is essential as an energy source, or fuel, from which the cells can generate ATP to carry out their particular energy-dependent activities such as contraction, transport, synthesis, secretion and even renewal of body tissues. Three primary categories of food ingested by humans which are carbohydrates, proteins

Enzymes Enzymes are catalysts. Most are proteins. (A few ribonucleoprotein enzymes have been discovered and, for some of these, the catalytic activity is in the RNA part rather than the protein part. Link to discussion of these ribozymes.) Enzymes bind temporarily to one or more of the reactants of the reaction they catalyze. In doing so, they lower the amount of

Unknown bacteria determined to be Alcaligenes faecalis because of its morphological, physiological and metabolic properties. In a laboratory setting, it often becomes necessary to identify an unknown organism. In this experiment, researchers classified an unidentified bacterium based on its physical structure, colony morphology, optimal conditions and metabolic properties. A Gram stain using crystal violet, iodine, and safranin and a simple stain using methylene blue characterized the organism’s

An Investigation on the rate of reaction of the enzyme Catalase on the substrate Hydrogen peroxide. Plan Aim: To investigate the rate of the effect of Catalase on hydrogen peroxide. Introduction This investigation will be carried out to investigate the rate of reaction of the enzyme catalase on the substrate hydrogen peroxide. Enzymes are biological catalysts, which speed up the rate of reaction without being used up during the reaction, which take place in living organisms. They do this by

of different size beads on the activity of immobilised catalyse Aim: To find out how varying the size of beads containing yeast varies the rate of activity of catalase in a fermentation reaction of hydrogen peroxide with immobilised enzymes. A catalyst is a substance that speeds up the rate of a chemical reaction, without itself being used up in the process however it maybe affected physically i.e. degradation of the surface of the catalyst. Catalysts work by reducing