Hanna Kim Lab 3 - Cells Report_Sp24 (1)
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University of California, Berkeley *
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Course
1AL
Subject
Biology
Date
Feb 20, 2024
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5
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Lab Report
LAB 3: Cells
Bio 1AL Spring 2024
Last Name
Kim
First Name
Hanna
Lab Section #
415
Lab 3 Report
1)
(1 pt) In the field of view circle below, draw an
Amoeba
at 200X total magnification (using the
20X objective).
a)
Label the following structures:
pseudopods, food vesicles
(and
contractile vacuole
if
observed
)
.
b)
Include a
scale bar
with
clearly labeled
length and units. (use reasonable values).
Note that
m (distance/length) is a different unit than
M (concentration).
µ
µ
Drawing instructions:
If you hand-drew the drawings, you can take a picture of the drawings
and insert the image below.
Total
Magnification: 200X
3-1
Lab Report
LAB 3: Cells
Bio 1AL Spring 2024
2)
(3 pt) In the left field of view circle below, draw a
Paramecium
cell and a few surrounding yeast
cells.
a)
Label the following structures:
cilia, contractile vacuole, yeast cell and oral groove
b)
Include a
scale bar
with clearly labeled length and units.
c)
Use the reticle calibration from Lab 2 to determine the length of the
Paramecium
, and the
diameter of a yeast cell. Include appropriate units in your answers in Table 1 below.
On the right field of view circle below, draw a stained
cheek cell
from your slide.
d)
Label the following structures:
nucleus, cell membrane, cytoplasm
e)
Include a
scale bar
with clearly labeled length and units (use reasonable values).
f)
Use the reticle calibration from Lab 2 to determine the widest part of the cheek cell, and the
diameter of the nucleus. Include appropriate units in your answers in Table 1 below.
Drawing instructions:
If you hand-drew the drawings, you can take a picture of the drawings and
insert the image below.
Total Magnification 400X
Total Magnification 400X
Table 1. Calculated Dimensions of Cellular Structures
Paramecium
Cheek Cell (measured
Total magnification: 400X
Total magnification: 400X
Length (long axis): 250um
Cheek cell diameter: 82.5um
Yeast cell diameter: 5 um
Nucleus diameter:
10 um
3)
Follow the Lab 3 procedures to measure rates of cytoplasmic streaming in
Nitella
. Record your
streaming rates below and on the class data sheet - use two decimals for your rates. For the class data
sheet, record your streaming rate in the corresponding green cells in the class data sheet by station
number.
3-2
Lab Report
LAB 3: Cells
Bio 1AL Spring 2024
Baseline rate (pre-FCCP)
Rate After FCCP Treatment
Experiment 1
93.02
2.37
Experiment 2
53.76
2.91
4a) Once all streaming times and streaming rates have been entered for all lab station groups in your lab
section, download your class datasheet as an Excel File (
File > Download > Microsoft Excel
).
4b) In Excel, calculate the
average, standard deviation, and standard error of the mean
(See Excel
Graphing Resources page) for the cytoplasmic streaming rates before (baseline) and after FCCP
addition (cells highlighted
blue
), using your lab section’s class data. Copy the values for the
average
streaming rates and
SEM
into Table 2 below.
4c) Calculate the
percentage change
between the two means and enter into Table 2. See the Comparing
Two Means page in bCourses.
4d) Run a
Correlated (Paired) Sample t-test
in VassarStats comparing the average baseline cytoplasmic
streaming rate to the average streaming rate after FCCP addition. See the Paired/Correlated Sample
t-test page in bCourses. Use the
one-tailed p-value
, since we are predicting that FCCP will decrease
cytoplasmic streaming rate (i.e. we are predicting an effect in one specific direction). Record the
relevant values in Table 2 below.
Table 2. Effect of FCCP on cytoplasmic streaming rate in
Nitella
Baseline
After FCCP
Mean streaming rate ± SEM (μm/sec)
43.8 ± 2.7
10.6 ± 1.4
Percentage change (%)
-75.9%
Paired t-test results
t = +10.24, df = 27, p (1-tailed) < 0.0001
5a) (0.5 pt) What is your null hypothesis for the t-test?
There is no statistically significant difference between baseline rates of cytoplasmic streaming and
the rates of cytoplasmic streaming after the addition of FCCP, and any differences are due to random
chance.
5b) (0.5 pt) Based on the results of your t-test, does FCCP addition significantly decrease cytoplasmic
streaming rate?
Circle/highlight:
YES
/ NO
6)
(1.5 pt) Using Excel, graph the average cytoplasmic streaming rate before (baseline) and after FCCP
addition from the class data. Use a column graph in Excel, and add custom error bars, using your
calculated SEM values. Save the graph in Excel as an image (Ctrl Click on the graph >
Save as
3-3
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Plate 2
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